Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages Via PAI-1 and IL-8 Production

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Sep 10

PMID 34502382

Citations 19

Authors

Kazuki Kai

Masafumi Moriyama

A S M Rafiul Haque

Taichi Hattori

Akira Chinju

Chen Hu

Keigo Kubota

Yuka Miyahara

Noriko Kakizoe-Ishiguro

Shintaro Kawano

Seiji Nakamura

Affiliations

Soon will be listed here.

Abstract

Tumor-associated macrophages (TAMs) promote cancer cell proliferation and metastasis, as well as anti-tumor immune suppression. Recent studies have shown that tumors enhance the recruitment and differentiation of TAMs, but the detailed mechanisms have not been clarified. We thus examined the influence of cancer cells on the differentiation of monocytes to TAM subsets, including CD163, CD204, and CD206 cells, in oral squamous cell carcinoma (OSCC) using immunohistochemistry, flow cytometry, and a cytokine array. Furthermore, we investigated the effect of OSCC cells (HSC-2, SQUU-A, and SQUU-B cells) on the differentiation of purified CD14 cells to TAM subsets. The localization patterns of CD163, CD204, and CD206 in OSCC sections were quite different. The expression of CD206 on CD14 cells was significantly increased after the co-culture with OSCC cell lines, while the expressions of CD163 and CD204 on CD14 cells showed no change. High concentrations of plasminogen activator inhibitor-1 (PAI-1) and interleukin-8 (IL-8) were detected in the conditioned medium of OSCC cell lines. PAI-1 and IL-8 stimulated CD14 cells to express CD206. Moreover, there were positive correlations among the numbers of CD206, PAI-1, and IL-8 cells in OSCC sections. These results suggest that PAI-1 and IL-8 produced by OSCC contribute to the differentiation of monocytes to CD206 TAMs.

Citing Articles

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Reciprocal Interactions of Human Monocytes and Cancer Cells in Co-Cultures In Vitro.

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