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Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus-Oocyte Complex Activity Through TLR4-Derived ERK1/2 Signaling in Mice

Overview
Journal Int J Mol Sci
Publisher MDPI
Date 2021 Sep 10
PMID 34502346
Citations 6
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Abstract

Peroxiredoxins (PRDXs) are expressed in the ovary and during ovulation. PRDX1 activity related to the immuno-like response during ovulation is unknown. We investigated the roles of on TLR4 and ERK1/2 signaling from the ovulated cumulus-oocyte complex (COC) using -knockout (K/O) and wild-type (WT) mice. Ovulated COCs were collected 12 and 16 h after pregnant mare serum gonadotropin/hCG injection. PRDX1 protein expression and COC secretion factors (, , and ) increased 16 h after ovulated COCs of the WT mice were obtained. We treated the ovulated COCs in mice with LPS (0.5 μg/mL) or hyaluronidase (Hya) (10 units/mL) to induce TLR4 activity. Intracellular reactive oxygen species (ROS), cumulus cell apoptosis, PRDX1, TLR4/P38/ERK1/2 protein expression, and COC secretion factors' mRNA levels increased in LPS- and Hya-treated COCs. The ERK inhibitor (U0126) and siRNA affected TLR4/ERK1/2 expression. The number and cumulus expansion of ovulated COCs by ROS were impaired in K/O mice but not in WT ones. gene deletion induced TLR4/P38/ERK1/2 expression and cumulus expansion genes. These results show the controlling roles of PRDX1 for TLR4/P38/ERK1/2 signaling activity in ovulated mice and the interlink of COCs with ovulation.

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References
1.
Marei W, Salavati M, Fouladi-Nashta A . Critical role of hyaluronidase-2 during preimplantation embryo development. Mol Hum Reprod. 2013; 19(9):590-9. DOI: 10.1093/molehr/gat032. View

2.
Samir M, Glister C, Mattar D, Laird M, Knight P . Follicular expression of pro-inflammatory cytokines tumour necrosis factor-α (TNFα), interleukin 6 (IL6) and their receptors in cattle: TNFα, IL6 and macrophages suppress thecal androgen production . Reproduction. 2017; 154(1):35-49. DOI: 10.1530/REP-17-0053. View

3.
McCubrey J, Lahair M, Franklin R . Reactive oxygen species-induced activation of the MAP kinase signaling pathways. Antioxid Redox Signal. 2006; 8(9-10):1775-89. DOI: 10.1089/ars.2006.8.1775. View

4.
Takeo T, Nakagata N . Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PLoS One. 2015; 10(5):e0128330. PMC: 4449130. DOI: 10.1371/journal.pone.0128330. View

5.
Girish K, Kemparaju K . The magic glue hyaluronan and its eraser hyaluronidase: a biological overview. Life Sci. 2007; 80(21):1921-43. DOI: 10.1016/j.lfs.2007.02.037. View