In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Sep 10

PMID 34502243

Citations 8

Authors

Petra Chocholata

Vlastimil Kulda

Jana Dvorakova

Monika Supova

Margit Zaloudkova

Vaclav Babuska

Affiliations

Soon will be listed here.

Abstract

Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion ( = 0.0440), as well as the proliferation in the following days ( < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.

Citing Articles

Tri-Component Hydrogel as Template for Nanocrystalline Hydroxyapatite Deposition Using Alternate Soaking Method for Bone Tissue Engineering Applications.

Pelin I, Popescu I, Calin M, Rebleanu D, Voicu G, Ionita D Gels. 2023; 9(11).

PMID: 37998995 PMC: 10671408. DOI: 10.3390/gels9110905.

Optimizing PCL/PLGA Scaffold Biocompatibility Using Gelatin from Bovine, Porcine, and Fish Origin.

Ghafouri Azar M, Wiesnerova L, Dvorakova J, Chocholata P, Moztarzadeh O, Dejmek J Gels. 2023; 9(11).

PMID: 37998990 PMC: 10670940. DOI: 10.3390/gels9110900.

Research advances of nanomaterials for the acceleration of fracture healing.

Zhang M, Xu F, Cao J, Dou Q, Wang J, Wang J Bioact Mater. 2023; 31:368-394.

PMID: 37663621 PMC: 10474571. DOI: 10.1016/j.bioactmat.2023.08.016.

Incorporation/Enrichment of 3D Bioprinted Constructs by Biomimetic Nanoparticles: Tuning Printability and Cell Behavior in Bone Models.

Fischetti T, Borciani G, Avnet S, Rubini K, Baldini N, Graziani G Nanomaterials (Basel). 2023; 13(14).

PMID: 37513050 PMC: 10386079. DOI: 10.3390/nano13142040.

Synthesis and Characterization of Epigallocatechin Gallate-mediated Hydroxyapatite.

Dinesh H, Sundar S, Kannan S, Ramadoss R, Selvam S, Ramani P Pharm Nanotechnol. 2023; 12(2):165-170.

PMID: 37287296 DOI: 10.2174/2211738511666230607113610.

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