Biochar-Enhanced Resistance to in Strawberry Fruits (But Not Leaves) Is Associated With Changes in the Rhizosphere Microbiome

Overview

Journal Front Plant Sci

Date 2021 Sep 9

PMID 34497619

Citations 5

Authors

Caroline De Tender

Bart Vandecasteele

Bruno Verstraeten

Sarah Ommeslag

Tina Kyndt

Jane Debode

Affiliations

Soon will be listed here.

Abstract

Biochar has been reported to play a positive role in disease suppression against airborne pathogens in plants. The mechanisms behind this positive trait are not well-understood. In this study, we hypothesized that the attraction of plant growth-promoting rhizobacteria (PGPR) or fungi (PGPF) underlies the mechanism of biochar in plant protection. The attraction of PGPR and PGPF may either activate the innate immune system of plants or help the plants with nutrient uptake. We studied the effect of biochar in peat substrate (PS) on the susceptibility of strawberry, both on leaves and fruits, against the airborne fungal pathogen . Biochar had a positive impact on the resistance of strawberry fruits but not the plant leaves. On leaves, the infection was more severe compared with plants without biochar in the PS. The different effects on fruits and plant leaves may indicate a trade-off between plant parts. Future studies should focus on monitoring gene expression and metabolites of strawberry fruits to investigate this potential trade-off effect. A change in the microbial community in the rhizosphere was also observed, with increased fungal diversity and higher abundances of amplicon sequence variants classified into , , and surrounding the plant root, where the latter two were reported as biocontrol agents. The change in the microbial community was not correlated with a change in nutrient uptake by the plant in either the leaves or the fruits. A decrease in the defense gene expression in the leaves was observed. In conclusion, the decreased infection of in strawberry fruits mediated by the addition of biochar in the PS is most likely regulated by the changes in the microbial community.

Citing Articles

Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against wilt.

Jin X, Bai Y, Khashi U Rahman M, Kang X, Pan K, Wu F Imeta. 2024; 1(3):e37.

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Secondary Metabolites and Their Role in Strawberry Defense.

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Garza-Alonso C, Olivares-Saenz E, Gonzalez-Morales S, Cabrera-De la Fuente M, Juarez-Maldonado A, Gonzalez-Fuentes J Plants (Basel). 2022; 11(24).

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References

Herschkovitz Y, Lerner A, Davidov Y, Okon Y, Jurkevitch E . Azospirillum brasilense does not affect population structure of specific rhizobacterial communities of inoculated maize (Zea mays). Environ Microbiol. 2005; 7(11):1847-52. DOI: 10.1111/j.1462-2920.2005.00926.x. View

Hirakawa H, Shirasawa K, Kosugi S, Tashiro K, Nakayama S, Yamada M . Dissection of the octoploid strawberry genome by deep sequencing of the genomes of Fragaria species. DNA Res. 2013; 21(2):169-81. PMC: 3989489. DOI: 10.1093/dnares/dst049. View

De Tender C, Vandecasteele B, Verstraeten B, Ommeslag S, De Meyer T, De Visscher J . Chitin in Strawberry Cultivation: Foliar Growth and Defense Response Promotion, but Reduced Fruit Yield and Disease Resistance by Nutrient Imbalances. Mol Plant Microbe Interact. 2020; 34(3):227-239. DOI: 10.1094/MPMI-08-20-0223-R. View

Jaiswal A, Elad Y, Paudel I, Graber E, Cytryn E, Frenkel O . Linking the Belowground Microbial Composition, Diversity and Activity to Soilborne Disease Suppression and Growth Promotion of Tomato Amended with Biochar. Sci Rep. 2017; 7:44382. PMC: 5347032. DOI: 10.1038/srep44382. View

Callahan B, McMurdie P, Rosen M, Han A, Johnson A, Holmes S . DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016; 13(7):581-3. PMC: 4927377. DOI: 10.1038/nmeth.3869. View

Mendes R, Garbeva P, Raaijmakers J . The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS Microbiol Rev. 2013; 37(5):634-63. DOI: 10.1111/1574-6976.12028. View

Vlot A, Dempsey D, Klessig D . Salicylic Acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol. 2009; 47:177-206. DOI: 10.1146/annurev.phyto.050908.135202. View

Nunes-Nesi A, Alseekh S, Silva F, Omranian N, Lichtenstein G, Mirnezhad M . Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds. Metabolomics. 2019; 15(4):46. PMC: 6420416. DOI: 10.1007/s11306-019-1503-8. View

De Tender C, Mesuere B, Van der Jeugt F, Haegeman A, Ruttink T, Vandecasteele B . Peat substrate amended with chitin modulates the N-cycle, siderophore and chitinase responses in the lettuce rhizobiome. Sci Rep. 2019; 9(1):9890. PMC: 6617458. DOI: 10.1038/s41598-019-46106-x. View

10.

Bakker P, Doornbos R, Zamioudis C, Berendsen R, Pieterse C . Induced systemic resistance and the rhizosphere microbiome. Plant Pathol J. 2014; 29(2):136-43. PMC: 4174772. DOI: 10.5423/PPJ.SI.07.2012.0111. View

11.

Jaiswal A, Elad Y, Cytryn E, Graber E, Frenkel O . Activating biochar by manipulating the bacterial and fungal microbiome through pre-conditioning. New Phytol. 2018; 219(1):363-377. DOI: 10.1111/nph.15042. View

12.

Jacoby R, Koprivova A, Kopriva S . Pinpointing secondary metabolites that shape the composition and function of the plant microbiome. J Exp Bot. 2020; 72(1):57-69. PMC: 7816845. DOI: 10.1093/jxb/eraa424. View

13.

Samain E, Aussenac T, Selim S . The Effect of Plant Genotype, Growth Stage, and Strains on the Efficiency and Durability of Wheat-Induced Resistance by sp. Strain B2. Front Plant Sci. 2019; 10:587. PMC: 6521617. DOI: 10.3389/fpls.2019.00587. View

14.

Philippot L, Raaijmakers J, Lemanceau P, van der Putten W . Going back to the roots: the microbial ecology of the rhizosphere. Nat Rev Microbiol. 2013; 11(11):789-99. DOI: 10.1038/nrmicro3109. View

15.

Wang W, Wang Z, Yang K, Wang P, Wang H, Guo L . Biochar Application Alleviated Negative Plant-Soil Feedback by Modifying Soil Microbiome. Front Microbiol. 2020; 11:799. PMC: 7201025. DOI: 10.3389/fmicb.2020.00799. View

16.

Lawrence M, Huber W, Pages H, Aboyoun P, Carlson M, Gentleman R . Software for computing and annotating genomic ranges. PLoS Comput Biol. 2013; 9(8):e1003118. PMC: 3738458. DOI: 10.1371/journal.pcbi.1003118. View

17.

Huang W, Ji H, Gheysen G, Debode J, Kyndt T . Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections. BMC Plant Biol. 2015; 15:267. PMC: 4632470. DOI: 10.1186/s12870-015-0654-7. View

18.

Pieterse C, Zamioudis C, Berendsen R, Weller D, Van Wees S, Bakker P . Induced systemic resistance by beneficial microbes. Annu Rev Phytopathol. 2014; 52:347-75. DOI: 10.1146/annurev-phyto-082712-102340. View

19.

Elad Y, David D, Meller Harel Y, Borenshtein M, Kalifa H, Silber A . Induction of systemic resistance in plants by biochar, a soil-applied carbon sequestering agent. Phytopathology. 2010; 100(9):913-21. DOI: 10.1094/PHYTO-100-9-0913. View

20.

Madhaiyan M, Poonguzhali S, Lee J, Senthilkumar M, Lee K, Sundaram S . Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J Syst Evol Microbiol. 2009; 60(Pt 10):2451-2457. DOI: 10.1099/ijs.0.018713-0. View