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All-flesh Fruit in Tomato is Controlled by Reduced Expression Dosage of AFF Through a Structural Variant Mutation in the Promoter

Overview
Journal J Exp Bot
Specialty Biology
Date 2021 Sep 7
PMID 34490889
Citations 10
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Abstract

The formation of locule gel is an important process in tomato and is a typical characteristic of berry fruit. In this study, we examined a natural tomato mutant that produces all-flesh fruit (AFF) in which the locule tissue remains in a solid state during fruit development. We constructed different genetic populations to fine-map the causal gene for this trait and identified SlMBP3 as the locus conferring the locule gel formation, which we rename as AFF. We determined the causal mutation as a 416-bp deletion in the promoter region of AFF, which reduces its expression dosage. Generally, this sequence is highly conserved among Solanaceae, as well as within the tomato germplasm. Using BC6 near-isogenic lines, we determined that the reduced expression dosage of AFF did not affect the normal development of seeds, whilst producing unique, non-liquefied locule tissue that was distinct from that of normal tomatoes in terms of metabolic components. Combined analysis using mRNA-seq and metabolomics indicated the importance of AFF in locule tissue liquefaction. Our findings provide insights into fruit-type differentiation in Solanaceae crops and also present the basis for future applications of AFF in tomato breeding programs.

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References
1.
Kumar R, Khurana A . Functional genomics of tomato: opportunities and challenges in post-genome NGS era. J Biosci. 2014; 39(5):917-29. DOI: 10.1007/s12038-014-9480-6. View

2.
Seymour G, Poole M, Manning K, King G . Genetics and epigenetics of fruit development and ripening. Curr Opin Plant Biol. 2007; 11(1):58-63. DOI: 10.1016/j.pbi.2007.09.003. View

3.
Ying S, Su M, Wu Y, Zhou L, Fu R, Li Y . Trichome regulator SlMIXTA-like directly manipulates primary metabolism in tomato fruit. Plant Biotechnol J. 2019; 18(2):354-363. PMC: 6953195. DOI: 10.1111/pbi.13202. View

4.
Azzi L, Deluche C, Gevaudant F, Frangne N, Delmas F, Hernould M . Fruit growth-related genes in tomato. J Exp Bot. 2015; 66(4):1075-86. DOI: 10.1093/jxb/eru527. View

5.
Vrebalov J, Pan I, Arroyo A, McQuinn R, Chung M, Poole M . Fleshy fruit expansion and ripening are regulated by the Tomato SHATTERPROOF gene TAGL1. Plant Cell. 2009; 21(10):3041-62. PMC: 2782289. DOI: 10.1105/tpc.109.066936. View