In Vitro Anthelmintic Activity of and Essential Oils on Gastrointestinal Nematodes from Sheep and Cattle

Overview

Journal J Parasit Dis

Specialty Parasitology

Date 2021 Sep 3

PMID 34475637

Citations 1

Authors

Gerardo Jimenez-Penago

Roberto Gonzalez-Garduno

Luciano Martinez-Bolanos

Ema Maldonado-Siman

Alvar A Cruz-Tamayo

Pedro Mendoza-de Gives

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to evaluate the in vitro nematicide activity of the allspice () and oregano () essential oils (EOs) on eggs and larvae of gastrointestinal nematodes (GIN) from ruminant. The EOs were obtained by hydro-distillation of previously dehydrated plant material. In the in vitro evaluation, the egg hatching and larval mobility of and spp. of sheep and cattle, respectively, were determined. EOs at concentrations of 20.2-252.3 µg µL with serial dilutions were used, also a negative control (distilled water + Tween 20) and Thiabendazol (77 µg µL) and Levamisole (120 µg µL) as a positive control to eggs and larvae, respectively, were placed on microplates. The results were analyzed with the PROBIT procedure. PPEO showed ovicidal effect in vitro test, with lethal doses 99 (LD) of 0.56-4.19 µg µL, while oregano essential oil (OREO) was shown to be a larvicidal activity with doses of LD of 0.15-6.60 L. The results confirm that and EOs have ovicidal and larvicidal capacity against GIN in vitro test.

Citing Articles

A Potential Anthelmintic Phytopharmacological Source of (L.) Essential Oil against Gastrointestinal Nematodes of Sheep.

Strbac F, Krnjajic S, Maurelli M, Stojanovic D, Simin N, Orcic D Animals (Basel). 2023; 13(1).

PMID: 36611652 PMC: 9817997. DOI: 10.3390/ani13010045.

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