Serum Exosome-derived Biomarkers for the Early Detection of Oral Squamous Cell Carcinoma

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2021 Sep 1

PMID 34468926

Citations 6

Authors

Hejia Guo

Weidong Jiang

Suhua Huang

XuanPing Huang

Cuiping Li

Affiliations

Soon will be listed here.

Abstract

Blood exosomes help regulate communication between tumour cells, moderating their behaviour. We sought to determine the protein content in serum exosomes (SEs), to characterise SEs, and to discover novel clinical biomarkers of oral squamous cell carcinoma (OSCC). Differentially expressed proteins (DEPs) of OSCC were identified using proteomics and then analysed using bioinformatics, before validation using ELISA, IHC, and RT-PCR. The influence of SEs on oral cancer cells was detected using CCK-8 and migration assays. Twelve DEPs were found in SEs from OSCC. Four proteins were targeted for further verification. New biomarkers exhibiting high sensitivity and specificity in diagnosing OSCC comprised C-reactive protein (CRP), von willebrand factor (VWF), and leucine-rich alpha-2-glycoprotein (LRG). Combined biomarkers outperformed any single protein. We also demonstrated that tumour-derived exosomes promoted tumour cell migration, but not proliferation and apoptosis. Our study indicates that CRP, VWF, and LRG are potential clinically relevant OSCC biomarkers. OSCC-related SEs may help promote migration of oral cells.

Citing Articles

Exosomes in Oral Diseases: Mechanisms and Therapeutic Applications.

Miao Q, Li S, Lyu W, Zhang J, Han Y Drug Des Devel Ther. 2025; 19:457-469.

PMID: 39867866 PMC: 11766710. DOI: 10.2147/DDDT.S505355.

Emerging roles of exosomes in oral diseases progression.

Wang J, Jing J, Zhou C, Fan Y Int J Oral Sci. 2024; 16(1):4.

PMID: 38221571 PMC: 10788352. DOI: 10.1038/s41368-023-00274-9.

Small Extracellular Vesicle-Derived vWF Induces a Positive Feedback Loop between Tumor and Endothelial Cells to Promote Angiogenesis and Metastasis in Hepatocellular Carcinoma.

Wong S, Tey S, Mao X, Fung H, Xiao Z, Wong D Adv Sci (Weinh). 2023; 10(26):e2302677.

PMID: 37387563 PMC: 10502836. DOI: 10.1002/advs.202302677.

Liquid Biopsy and Circulating Biomarkers for the Diagnosis of Precancerous and Cancerous Oral Lesions.

Gattuso G, Crimi S, Lavoro A, Rizzo R, Musumarra G, Gallo S Noncoding RNA. 2022; 8(4).

PMID: 36005828 PMC: 9414906. DOI: 10.3390/ncrna8040060.

Current Status, Opportunities, and Challenges of Exosomes in Oral Cancer Diagnosis and Treatment.

Liu H, Huang Y, Huang M, Huang Z, Wang Q, Qing L Int J Nanomedicine. 2022; 17:2679-2705.

PMID: 35733418 PMC: 9208818. DOI: 10.2147/IJN.S365594.

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