Measurements of Free-swimming Speed of Motile Cells in Liquid Media

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Aug 30

PMID 34458423

Citations 5

Authors

Yusuke V Morimoto

Keiichi Namba

Tohru Minamino

Affiliations

Soon will be listed here.

Abstract

Bacteria such as and swim in liquid media using the bacterial flagella. The flagellum consists of the basal body (rotary motor), the hook (universal joint) and the filament (helical screw). Since mutants with a defect in flagellar assembly and function cannot swim smoothly, motility assay is an easy way to characterize flagellar mutants. Here, we describe how to measure free-swimming speeds of motile cells in liquid media. Free-swimming behavior under a microscope shows a significant variation among bacterial cells.

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GFP Fusion to the N-Terminus of MotB Affects the Proton Channel Activity of the Bacterial Flagellar Motor in .

Morimoto Y, Namba K, Minamino T Biomolecules. 2020; 10(9).

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Novel Insights into Conformational Rearrangements of the Bacterial Flagellar Switch Complex.

Sakai T, Miyata T, Terahara N, Mori K, Inoue Y, Morimoto Y mBio. 2019; 10(2).

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References

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