Transcriptomic Reprograming of Pv. After Treatment with Hydrolytic Products Derived from Glucosinolates

Overview

Journal Plants (Basel)

Date 2021 Aug 28

PMID 34451701

Citations 1

Authors

Pari Madloo

Margarita Lema

Victor Manuel Rodriguez

Pilar Soengas

Affiliations

Soon will be listed here.

Abstract

The bacterium pv. () causes black rot disease in crops. Glucosinolates are known to be part of the defence system of crops against infection. They are activated upon pathogen attack by myrosinase enzymes. Their hydrolytic products (GHPs) inhibit the growth of in vitro. However, the mechanisms underlying this inhibition and the way can overcome it are not well understood. We studied the transcriptomic reprogramming of after being supplemented with two chemically different GHPs, one aliphatic isothiocyanate (allyl-ITC) and one indole (indol-3-carbinol), by RNA-seq. Based on our results, the arrest in growth is related to the need to stop cell division to repair damaged DNA and cell envelope components. Otherwise, GHPs modify energy metabolism by inhibiting aerobic respiration and increasing the synthesis of glycogen. induces detoxification mechanisms such as the antioxidant defence system and the multidrug efflux system to cope with the toxic effects driven by GHPs. This is the first time that the transcriptomic reprogramming of a plant pathogenic bacterium treated with GHPs has been studied. This information will allow a better understanding of the interaction of a plant pathogen mediated by GSLs.

Citing Articles

Constructing a Novel Disease Resistance Mechanism Model for Cruciferous Crops: An Example From Black Rot.

Dai H, Hu L, Wang J, Yue Z, Wang J, Chen T Mol Plant Pathol. 2025; 26(2):e70060.

PMID: 39924905 PMC: 11808048. DOI: 10.1111/mpp.70060.

Antagonistic transcriptome profile reveals potential mechanisms of action on pv. by the cell-free supernatants of 504, a versatile plant probiotic bacterium.

Zhou Q, Tu M, Fu X, Chen Y, Wang M, Fang Y Front Cell Infect Microbiol. 2023; 13:1175446.

PMID: 37325518 PMC: 10265122. DOI: 10.3389/fcimb.2023.1175446.

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