Bioactivity-Guided Extract Optimization of Var. Leaves and Anti-Inflammatory Activities of Phillyrin

Overview

Journal Plants (Basel)

Date 2021 Aug 28

PMID 34451589

Citations 6

Authors

Hwa-Young Song

Da-Eun Jeong

Mina Lee

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to identify the optimal extraction conditions for leaves of var. . Inhibitory effects of various extracts on NO production were compared. Antioxidant evaluations for total phenol and flavonoid contents were carried out using various extracts of var. leaves obtained under optimal extraction conditions that showed the greatest effect on NO production. The optimal method for extracting var. leaves resulted in an extract named OP OFLE. OP OFLE showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. Phillyrin (PH) was isolated as a major compound from OP OFLE by HPLC/DAD analysis. OP OFLE and PH reduced inducible nitric oxide (iNOS) and cyclooxygenase (COX)-2 protein expression and downregulated proinflammatory cytokines such as interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 and HT-29 cells. To determine the signal pathway involved in the inhibition of NO production, a Western blot analysis was performed. Results showed that OP OFLE decreased phosphorylation of extracellular regulated kinase (pERK) 1/2 and the expression of nuclear factor-kappa B (NF-κB). Our results suggest that extracts of var. leaves and its major components have biological activities such as antioxidative and anti-inflammatory properties.

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