Molecular Confirmation of Massive Cysticercosis in One Rabbit in Poland

Overview

Journal Pathogens

Date 2021 Aug 28

PMID 34451493

Citations 5

Authors

Malgorzata Samorek-Pierog

Jacek Karamon

Adam Brzana

Ewa Bilska-Zajac

Jolanta Zdybel

Tomasz Cencek

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to provide molecular characterization, together with phylogenetic analysis, of cysts isolated from rabbit. On the basis of morphological features and molecular analysis, the cysticerci were identified as metacestodes. PCR was performed with three different protocols to obtain partial sequences of 12S ribosomal RNA (12S rRNA), NADH dehydrogenase subunit 1 (nad1), and cytochrome oxidase subunit 1 (cox1) of spp. The products from the PCRs were sequenced. Interpretation of the sequencing results of the obtained amplicons, by comparing them with the GenBank database, proved that the causative agent, in this case, was . The phylogenetic analysis of the received sequences identified a new haplotype. The received data can be used to supplement the species description. To our knowledge, this is the first molecular confirmation of metacestodes infection in the rabbit, in Poland.

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References

Jenkins D, Lievaart J, Boufana B, Lett W, Bradshaw H, Armua-Fernandez M . Echinococcus granulosus and other intestinal helminths: current status of prevalence and management in rural dogs of eastern Australia. Aust Vet J. 2014; 92(8):292-8. DOI: 10.1111/avj.12218. View

Allan J, Craig P, Sherington J, Rogan M, Storey D, Heath S . Helminth parasites of the wild rabbit Oryctolagus cuniculus near Malham Tarn, Yorkshire, UK. J Helminthol. 2000; 73(4):289-94. DOI: 10.1017/s0022149x99000487. View

Foronda P, Del Castillo A, Abreu N, Figueruelo E, Pinero J, Casanova J . Parasitic helminths of the wild rabbit, Oryctolagus cuniculus, in different bioclimatic zones in Tenerife, Canary Islands. J Helminthol. 2003; 77(4):305-9. DOI: 10.1079/joh2003182. View

Trachsel D, Deplazes P, Mathis A . Identification of taeniid eggs in the faeces from carnivores based on multiplex PCR using targets in mitochondrial DNA. Parasitology. 2007; 134(Pt 6):911-20. DOI: 10.1017/S0031182007002235. View

Stancampiano L, Ravagnan S, Capelli G, Militerno G . Cysticercosis by in Brown Hare () in Northern Italy: Epidemiologic and pathologic features. Int J Parasitol Parasites Wildl. 2019; 9:139-143. PMC: 6506461. DOI: 10.1016/j.ijppaw.2019.04.004. View

Casulli A, Manfredi M, La Rosa G, Di Cerbo A, Genchi C, Pozio E . Echinococcus ortleppi and E. granulosus G1, G2 and G3 genotypes in Italian bovines. Vet Parasitol. 2008; 155(1-2):168-72. DOI: 10.1016/j.vetpar.2008.04.004. View

. An up-date of Verster's (1969) 'Taxonomic revision of the genus Taenia Linnaeus' (Cestoda) in table format. Syst Parasitol. 2000; 45(3):155-83. DOI: 10.1023/a:1006219625792. View

Zhang S . Comparative Transcriptomic Analysis of the Larval and Adult Stages of . Genes (Basel). 2019; 10(7). PMC: 6678355. DOI: 10.3390/genes10070507. View

Keith L, Cary J, Yuill T, Keith I . Prevalence of helminths in a cyclic snowshoe hare population. J Wildl Dis. 1985; 21(3):233-53. DOI: 10.7589/0090-3558-21.3.233. View

10.

Arias-Hernandez D, Garcia-Jimenez S, Dominguez-Roldan R, Murcia-Mejia C, Baez-Saldana A, Hallal-Calleros C . Effects of Infection and Obesity on Clinical Parameters, Organometry and Fat Distribution in Male Rabbits. Pathogens. 2020; 9(11). PMC: 7690263. DOI: 10.3390/pathogens9110861. View

11.

Dominguez-Roldan R, Perez-Martinez M, Rosetti M, Arias-Hernandez D, Bernal-Fernandez G, Flores-Perez F . High frequency of Taenia pisiformis metacestodes and high sex-associated susceptibility to cysticercosis in naturally infected wild rabbits. Parasitol Res. 2018; 117(7):2201-2206. DOI: 10.1007/s00436-018-5907-6. View

12.

Bowles J, Blair D, McManus D . Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-73. DOI: 10.1016/0166-6851(92)90109-w. View

13.

Luong L, Chambers J, Moizis A, Stock T, St Clair C . Helminth parasites and zoonotic risk associated with urban coyotes () in Alberta, Canada. J Helminthol. 2018; 94:e25. DOI: 10.1017/S0022149X1800113X. View

14.

Szkucik K, Pyz-Lukasik R, Szczepaniak K, Paszkiewicz W . Occurrence of gastrointestinal parasites in slaughter rabbits. Parasitol Res. 2013; 113(1):59-64. PMC: 3898139. DOI: 10.1007/s00436-013-3625-7. View

15.

Dybicz M, Borkowski P, Padzik M, Baltaza W, Chomicz L . Molecular determination of suspected alveolar echinococcosis requiring surgical treatment in human cases from Poland. Ann Parasitol. 2019; 64(4):339-342. View

16.

Mathis A, Deplazes P . Copro-DNA tests for diagnosis of animal taeniid cestodes. Parasitol Int. 2005; 55 Suppl:S87-90. DOI: 10.1016/j.parint.2005.11.012. View

17.

Trasvina-Munoz E, Lopez-Valencia G, Monge-Navarro F, Herrera-Ramirez J, Haro P, Gomez-Gomez S . Detection of Intestinal Parasites in Stray Dogs from a Farming and Cattle Region of Northwestern Mexico. Pathogens. 2020; 9(7). PMC: 7400657. DOI: 10.3390/pathogens9070516. View

18.

Bowles J, McManus D . NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol. 1993; 23(7):969-72. DOI: 10.1016/0020-7519(93)90065-7. View

19.

Hallal-Calleros C, Morales-Montor J, Orihuela-Trujillo A, Togno-Peirce C, Murcia-Mejia C, Bielli A . Taenia pisiformis cysticercosis induces decreased prolificacy and increased progesterone levels in rabbits. Vet Parasitol. 2016; 229:50-53. DOI: 10.1016/j.vetpar.2016.09.015. View

20.

Yang D, Ren Y, Fu Y, Xie Y, Nie H, Nong X . Genetic variation of Taenia pisiformis collected from Sichuan, China, based on the mitochondrial cytochrome B gene. Korean J Parasitol. 2013; 51(4):449-52. PMC: 3770876. DOI: 10.3347/kjp.2013.51.4.449. View