» Articles » PMID: 34451215

Preparation and Characterization of Salt-Mediated Injectable Thermosensitive Chitosan/Pectin Hydrogels for Cell Embedding and Culturing

Overview
Publisher MDPI
Date 2021 Aug 28
PMID 34451215
Citations 8
Authors
Affiliations
Soon will be listed here.
Abstract

In recent years, growing attention has been directed to the development of 3D in vitro tissue models for the study of the physiopathological mechanisms behind organ functioning and diseases. Hydrogels, acting as 3D supporting architectures, allow cells to organize spatially more closely to what they physiologically experience in vivo. In this scenario, natural polymer hybrid hydrogels display marked biocompatibility and versatility, representing valid biomaterials for 3D in vitro studies. Here, thermosensitive injectable hydrogels constituted by chitosan and pectin were designed. We exploited the feature of chitosan to thermally undergo sol-gel transition upon the addition of salts, forming a compound that incorporates pectin into a semi-interpenetrating polymer network (semi-IPN). Three salt solutions were tested, namely, beta-glycerophosphate (βGP), phosphate buffer (PB) and sodium hydrogen carbonate (SHC). The hydrogel formulations were injectable at room temperature, gelled at 37 °C and presented a physiological pH, suitable for cell encapsulation. Hydrogels were stable in culture conditions, were able to retain a high water amount and displayed an open and highly interconnected porosity and suitable mechanical properties, with Young's modulus values in the range of soft biological tissues. The developed chitosan/pectin system can be successfully used as a 3D in vitro platform for studying tissue physiopathology.

Citing Articles

Photo/thermo-sensitive chitosan and gelatin-based interpenetrating polymer network for mimicking muscle tissue extracellular matrix.

Stanzione A, Polini A, Scalera F, Gigli G, Moroni L, Gervaso F Heliyon. 2024; 10(21):e39820.

PMID: 39553568 PMC: 11567107. DOI: 10.1016/j.heliyon.2024.e39820.


The Evolution of Technology-Driven In Vitro Models for Neurodegenerative Diseases.

De Vitis E, Stanzione A, Romano A, Quattrini A, Gigli G, Moroni L Adv Sci (Weinh). 2024; 11(16):e2304989.

PMID: 38366798 PMC: 11040362. DOI: 10.1002/advs.202304989.


Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer.

Vigliar M, Marega L, Duarte M, Alcalde M, Rosso M, Ferreira Junior R Bioengineering (Basel). 2024; 11(1).

PMID: 38247955 PMC: 10813421. DOI: 10.3390/bioengineering11010078.


pH-sensing hybrid hydrogels for non-invasive metabolism monitoring in tumor spheroids.

Rizzo R, Onesto V, Morello G, Iuele H, Scalera F, Forciniti S Mater Today Bio. 2023; 20:100655.

PMID: 37234366 PMC: 10205545. DOI: 10.1016/j.mtbio.2023.100655.


Chitosan and Pectin Hydrogels for Tissue Engineering and In Vitro Modeling.

Morello G, De Iaco G, Gigli G, Polini A, Gervaso F Gels. 2023; 9(2).

PMID: 36826302 PMC: 9957157. DOI: 10.3390/gels9020132.


References
1.
Yang J, Zhang Y, Yue K, Khademhosseini A . Cell-laden hydrogels for osteochondral and cartilage tissue engineering. Acta Biomater. 2017; 57:1-25. PMC: 5545789. DOI: 10.1016/j.actbio.2017.01.036. View

2.
Hoffman A . Hydrogels for biomedical applications. Adv Drug Deliv Rev. 2002; 54(1):3-12. DOI: 10.1016/s0169-409x(01)00239-3. View

3.
Lapomarda A, De Acutis A, Chiesa I, Fortunato G, Montemurro F, De Maria C . Pectin-GPTMS-Based Biomaterial: toward a Sustainable Bioprinting of 3D scaffolds for Tissue Engineering Application. Biomacromolecules. 2019; 21(2):319-327. DOI: 10.1021/acs.biomac.9b01332. View

4.
Zanoni M, Cortesi M, Zamagni A, Arienti C, Pignatta S, Tesei A . Modeling neoplastic disease with spheroids and organoids. J Hematol Oncol. 2020; 13(1):97. PMC: 7364537. DOI: 10.1186/s13045-020-00931-0. View

5.
Shitrit Y, Davidovich-Pinhas M, Bianco-Peled H . Shear thinning pectin hydrogels physically cross-linked with chitosan nanogels. Carbohydr Polym. 2019; 225:115249. DOI: 10.1016/j.carbpol.2019.115249. View