Preliminary Report on the Acquisition, Persistence, and Potential Transmission of by

(CTV) is one of the most important citrus tree viruses: a graft-transmissible virus that can be vectored by several aphid species. is the insect vector of " Liberibacter spp.", a bacterium associated with citrus Huanglongbing (HLB). However, no detailed description of the relationship between CTV and has been reported. In this study, adults collected from CTV-infected "Shatangju" mandarin, "Newhall" sweet orange, and "fingered citron" trees in different orchards yielded CTV-positive rates of 40%, 65%, and 95%, respectively, upon detection by conventional PCR. Illumina HiSeq sequencing followed by assembly recovered the primary full CTV genome from the RNA of 30 adults sampled from CTV-positive citrus plants. Molting and adult emergence did not affect the presence or titers of CTV within the ; however, the persistence of CTV in psyllids varied among different host plant species. Groups of 10 (from a population 85% CTV-positive) were shown to potentially transmit CTV to two citrus species, "Shatangju" mandarin and "Eureka" lemon, yielding 58.33% and 83.33% CTV-positive plants, respectively. No transmission of CTV to orange jasmine plants occurred. Thus, this study reports on the ability of to acquire and transmit CTV, making as a vector of two important citrus pathogens, warranting further attention and investigation.