Predictive Antibiotic Susceptibility Testing by Next-Generation Sequencing for Periprosthetic Joint Infections: Potential and Limitations

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2021 Aug 27

PMID 34440114

Citations 11

Authors

Lukas Luftinger

Ines Ferreira

Bernhard J H Frank

Stephan Beisken

Johannes Weinberger

Arndt von Haeseler

Thomas Rattei

Jochen G Hofstaetter

Andreas E Posch

Arne Materna

Affiliations

Soon will be listed here.

Abstract

Joint replacement surgeries are one of the most frequent medical interventions globally. Infections of prosthetic joints are a major health challenge and typically require prolonged or even indefinite antibiotic treatment. As multidrug-resistant pathogens continue to rise globally, novel diagnostics are critical to ensure appropriate treatment and help with prosthetic joint infections (PJI) management. To this end, recent studies have shown the potential of molecular methods such as next-generation sequencing to complement established phenotypic, culture-based methods. Together with advanced bioinformatics approaches, next-generation sequencing can provide comprehensive information on pathogen identity as well as antimicrobial susceptibility, potentially enabling rapid diagnosis and targeted therapy of PJIs. In this review, we summarize current developments in next generation sequencing based predictive antibiotic susceptibility testing and discuss potential and limitations for common PJI pathogens.

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