Development of Two Loop-mediated Isothermal Amplification (LAMP) Genomics-informed Diagnostic Protocols for Rapid Detection of Species on Rice

Overview

Journal MethodsX

Specialty Pathology

Date 2021 Aug 26

PMID 34434739

Citations 2

Authors

Kossi Kini

Issa Wonni

Drissa Silue

Ralf Koebnik

Affiliations

Soon will be listed here.

Abstract

At least three species of are responsible for bacterial blight disease and grain discoloration of rice in Sub-Saharan Africa. Thus, measures need to be taken to limit the pathogens' dispersion and robust diagnostic tools are required for rapid and cheap diagnosis in the field as well as for routine seed certification or control. Therefore, several diagnostic tools such as simplex and multiplex PCR schemes and a semi-selective medium have been developed and are being used. However, the use of these tools is time-consuming, expensive and therefore limited to laboratories that can afford the chemicals. We have therefore developed two isothermal loop amplification (LAMP) protocols, one of which detects all species in the genus and another one that is specific for .•The novel LAMP assays allow rapid and sensitive detection of these bacteria.•They will help plant protection services in routine field and laboratory tests especially for monitoring the phytosanitary status of rice seeds.

Citing Articles

LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives.

Aglietti C, Benigno A, Cacciola S, Moricca S Life (Basel). 2025; 14(12.

PMID: 39768257 PMC: 11678381. DOI: 10.3390/life14121549.

Development of a Genome-Informed Protocol for Detection of pv. Using LAMP and PCR.

Diaz D, Zamorano A, Garcia H, Ramos C, Cui W, Carreras C Plants (Basel). 2023; 12(24).

PMID: 38140446 PMC: 10747947. DOI: 10.3390/plants12244119.

Detection and Control of Causing Plum Bacterial Shot-Hole Disease by Loop-Mediated Isothermal Amplification Technique.

Shu R, Yin X, Long Y, Yuan J, Zhou H Front Microbiol. 2022; 13:896567.

PMID: 35694300 PMC: 9175033. DOI: 10.3389/fmicb.2022.896567.

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