The Radish Ogura Fertility Restorer Impedes Translation Elongation Along Its Cognate CMS-causing MRNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2021 Aug 26

PMID 34433671

Citations 22

Authors

Chuande Wang

Lina Lezhneva

Nadege Arnal

Martine Quadrado

Hakim Mireau

Affiliations

Soon will be listed here.

Abstract

The control of messenger RNA (mRNA) translation has been increasingly recognized as a key regulatory step for gene control, but clear examples in eukaryotes are still scarce. Nucleo-cytoplasmic male sterilities (CMS) represent ideal genetic models to dissect genetic interactions between the mitochondria and the nucleus in plants. This trait is determined by specific mitochondrial genes and is associated with a pollen sterility phenotype that can be suppressed by nuclear genes known as restorer-of-fertility (). In this study, we focused on the Ogura CMS system in rapeseed and showed that reversion to male sterility by the PPR-B fertility restorer (also called Rfo) occurs through a specific translation inhibition of the mitochondria-encoded CMS-causing mRNA We also demonstrate that PPR-B binds within the coding sequence of and acts as a ribosome blocker to specifically impede translation elongation along the mRNA. Rfo is the first recognized fertility restorer shown to act this way. These observations will certainly facilitate the development of synthetic fertility restorers for CMS systems in which efficient natural Rfs are lacking.

Citing Articles

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Identification and variation of a new restorer of fertility gene that induces cleavage in orf138 mRNA of Ogura male sterility in radish.

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