MicroRNA Expression Profile in Lampetra Morii Upon Vibrio Anguillarum Infection and MiR-4561 Characterization Targeting Lip

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Aug 21

PMID 34417547

Citations 1

Authors

Lie Ma

Meng Gou

Zeyu Du

Ting Zhu

Jun Li

Qing Wei Li

Yue Pang

Affiliations

Soon will be listed here.

Abstract

As a critical evolutionary pivot between invertebrates and vertebrates, lampreys provide rich genetic information. Lamprey immune protein (LIP) is a key immune regulator. MicroRNAs, well-conserved in the response to immunological stress, remain understudied in lamprey immunity. We generated a lamprey microRNA expression atlas, using deep sequencing, upon Vibrio anguillarum infection. Using comparative methods, we found that miR-4561 potentially regulates innate immunity via interaction with lip. We found a sequence in the 3'-UTR region of LIP mRNA complementary to the miR-4561 seed region; miR-4561 expression was negatively correlated with LIP. During V. anguillarum infection, miR-4561 inhibited LIP expression and bacterial clearance. Notably, LIP expression in supraneural body cells was necessary for the Gram-negative immune response. Additionally, we observed that overexpression of miR-4561 induced apoptosis in embryonic cells, suggesting a role in embryonic development. Collectively, we show lamprey microRNAs may significantly affect gene regulation and provide new insights on LIP-mediated immune regulation.

Citing Articles

Lamprey immune protein triggers the ferroptosis pathway during zebrafish embryonic development.

Du Z, Zhang D, Li J, Li Q, Pang Y Cell Commun Signal. 2022; 20(1):124.

PMID: 35978430 PMC: 9386916. DOI: 10.1186/s12964-022-00933-0.

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