Excessive Treadmill Training Produces Different Cardiac-related MicroRNA Profiles in the Left and Right Ventricles in Mice

Overview

Journal Int J Sports Med

Publisher Thieme

Specialty Orthopedics

Date 2021 Aug 20

PMID 34416779

Citations 2

Authors

Jing Yang

Lin Xu

Xin Yin

Yi Li Zheng

Hai Peng Zhang

Sheng Jia Xu

Wei Wang

Sen Wang

Chen Yu Zhang

Ji Zheng Ma

Affiliations

Soon will be listed here.

Abstract

High-volume training followed by inadequate recovery may cause overtraining. This process may undermine the protective effect of regular exercise on the cardiovascular system and may increase the risk of pathological cardiac remodelling. We evaluated whether chronic overtraining changes cardiac-related microRNA profiles in the left and right ventricles. C57BL/6 mice were divided into the control, normal training, and overtrained by running without inclination, uphill running or downhill running groups. After an 8-week treadmill training protocol, the incremental load test and training volume results showed that the model had been successfully established. The qRT-PCR results showed increased cardiac miR-1, miR-133a, miR-133b, miR-206, miR-208b and miR-499 levels in the left ventricle of the downhill running group compared with the left ventricle of the control group. Similarly, compared with the control group, the downhill running induced increased expression of miR-21, miR-17-3p, and miR-29b in the left ventricle. Unlike the changes in the left ventricle, no difference in the expression of the tested miRNAs was observed in the right ventricle. Briefly, our results indicated that overtraining generally affects key miRNAs in the left ventricle (rather than the right ventricle) and that changes in individual miRNAs may cause either adaptive or maladaptive remodelling with overtraining.

Citing Articles

The Role and Potential Mechanisms of Rehabilitation Exercise Improving Cardiac Remodeling.

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PMID: 38558377 DOI: 10.1007/s12265-024-10498-7.

Left ventricle function and post-transcriptional events with exercise training in pigs.

Samani S, Barlow S, Freeburg L, Jones T, Poole M, Sarzynski M PLoS One. 2024; 19(2):e0292243.

PMID: 38306359 PMC: 10836705. DOI: 10.1371/journal.pone.0292243.

References

Meeusen R, Duclos M, Foster C, Fry A, Gleeson M, Nieman D . Prevention, diagnosis, and treatment of the overtraining syndrome: joint consensus statement of the European College of Sport Science and the American College of Sports Medicine. Med Sci Sports Exerc. 2012; 45(1):186-205. DOI: 10.1249/MSS.0b013e318279a10a. View

Matkovich S, Hu Y, Eschenbacher W, Dorn L, Dorn 2nd G . Direct and indirect involvement of microRNA-499 in clinical and experimental cardiomyopathy. Circ Res. 2012; 111(5):521-31. PMC: 3429338. DOI: 10.1161/CIRCRESAHA.112.265736. View

Schipper M, van Kuik J, de Jonge N, Dullens H, de Weger R . Changes in regulatory microRNA expression in myocardium of heart failure patients on left ventricular assist device support. J Heart Lung Transplant. 2008; 27(12):1282-5. DOI: 10.1016/j.healun.2008.09.005. View

Kuipers H, Verstappen F, Keizer H, Geurten P, Van Kranenburg G . Variability of aerobic performance in the laboratory and its physiologic correlates. Int J Sports Med. 1985; 6(4):197-201. DOI: 10.1055/s-2008-1025839. View

da Rocha A, Pereira B, Teixeira G, Pinto A, Frantz F, Elias L . Treadmill Slope Modulates Inflammation, Fiber Type Composition, Androgen, and Glucocorticoid Receptors in the Skeletal Muscle of Overtrained Mice. Front Immunol. 2017; 8:1378. PMC: 5669301. DOI: 10.3389/fimmu.2017.01378. View

Kambis T, Shahshahan H, Kar S, Yadav S, Mishra P . Transgenic Expression of miR-133a in the Diabetic Akita Heart Prevents Cardiac Remodeling and Cardiomyopathy. Front Cardiovasc Med. 2019; 6:45. PMC: 6491745. DOI: 10.3389/fcvm.2019.00045. View

Harriss D, MacSween A, Atkinson G . Ethical Standards in Sport and Exercise Science Research: 2020 Update. Int J Sports Med. 2019; 40(13):813-817. DOI: 10.1055/a-1015-3123. View

Dworatzek E, Mahmoodzadeh S, Schubert C, Westphal C, Leber J, Kusch A . Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta. Cardiovasc Res. 2014; 102(3):418-28. DOI: 10.1093/cvr/cvu065. View

Domanska-Senderowska D, Laguette M, Jegier A, Cieszczyk P, September A, Brzezianska-Lasota E . MicroRNA Profile and Adaptive Response to Exercise Training: A Review. Int J Sports Med. 2019; 40(4):227-235. DOI: 10.1055/a-0824-4813. View

10.

Yang Y, Del Re D, Nakano N, Sciarretta S, Zhai P, Park J . miR-206 Mediates YAP-Induced Cardiac Hypertrophy and Survival. Circ Res. 2015; 117(10):891-904. PMC: 4747867. DOI: 10.1161/CIRCRESAHA.115.306624. View

11.

Ma Z, Qi J, Meng S, Wen B, Zhang J . Swimming exercise training-induced left ventricular hypertrophy involves microRNAs and synergistic regulation of the PI3K/AKT/mTOR signaling pathway. Eur J Appl Physiol. 2013; 113(10):2473-86. DOI: 10.1007/s00421-013-2685-9. View

12.

Nogueira-Ferreira R, Ferreira R, Padrao A, Oliveira P, Santos M, Kavazis A . One year of exercise training promotes distinct adaptations in right and left ventricle of female Sprague-Dawley rats. J Physiol Biochem. 2019; 75(4):561-572. DOI: 10.1007/s13105-019-00705-4. View

13.

Morganroth J, Maron B, Henry W, Epstein S . Comparative left ventricular dimensions in trained athletes. Ann Intern Med. 1975; 82(4):521-4. DOI: 10.7326/0003-4819-82-4-521. View

14.

Ellison G, Waring C, Vicinanza C, Torella D . Physiological cardiac remodelling in response to endurance exercise training: cellular and molecular mechanisms. Heart. 2011; 98(1):5-10. DOI: 10.1136/heartjnl-2011-300639. View

15.

Hart E, Charkoudian N, Wallin B, Curry T, Eisenach J, Joyner M . Sex and ageing differences in resting arterial pressure regulation: the role of the β-adrenergic receptors. J Physiol. 2011; 589(Pt 21):5285-97. PMC: 3225680. DOI: 10.1113/jphysiol.2011.212753. View

16.

Fry A, Kraemer W . Resistance exercise overtraining and overreaching. Neuroendocrine responses. Sports Med. 1997; 23(2):106-29. DOI: 10.2165/00007256-199723020-00004. View

17.

La Gerche A, Burns A, Mooney D, Inder W, Taylor A, Bogaert J . Exercise-induced right ventricular dysfunction and structural remodelling in endurance athletes. Eur Heart J. 2011; 33(8):998-1006. DOI: 10.1093/eurheartj/ehr397. View

18.

Fernandes T, Barauna V, Negrao C, Phillips M, Oliveira E . Aerobic exercise training promotes physiological cardiac remodeling involving a set of microRNAs. Am J Physiol Heart Circ Physiol. 2015; 309(4):H543-52. PMC: 4537939. DOI: 10.1152/ajpheart.00899.2014. View

19.

Lindman B, Dweck M, Lancellotti P, Genereux P, Pierard L, OGara P . Management of Asymptomatic Severe Aortic Stenosis: Evolving Concepts in Timing of Valve Replacement. JACC Cardiovasc Imaging. 2019; 13(2 Pt 1):481-493. DOI: 10.1016/j.jcmg.2019.01.036. View

20.

Sucharov C, Bristow M, Port J . miRNA expression in the failing human heart: functional correlates. J Mol Cell Cardiol. 2008; 45(2):185-92. PMC: 2561965. DOI: 10.1016/j.yjmcc.2008.04.014. View