Characterization of the ABC Methionine Transporter from Reveals That Lipidated MetQ is Required for Interaction

Overview

Journal Elife

Specialty Biology

Date 2021 Aug 19

PMID 34409939

Citations 5

Authors

Naima G Sharaf

Mona Shahgholi

Esther Kim

Jeffrey Y Lai

David G VanderVelde

Allen T Lee

Douglas C Rees

Affiliations

Soon will be listed here.

Abstract

NmMetQ is a substrate-binding protein (SBP) from that has been identified as a surface-exposed candidate antigen for meningococcal vaccines. However, this location for NmMetQ challenges the prevailing view that SBPs in Gram-negative bacteria are localized to the periplasmic space to promote interaction with their cognate ABC transporter embedded in the bacterial inner membrane. To elucidate the roles of NmMetQ, we characterized NmMetQ with and without its cognate ABC transporter (NmMetNI). Here, we show that NmMetQ is a lipoprotein (lipo-NmMetQ) that binds multiple methionine analogs and stimulates the ATPase activity of NmMetNI. Using single-particle electron cryo-microscopy, we determined the structures of NmMetNI in the presence and absence of lipo-NmMetQ. Based on our data, we propose that NmMetQ tethers to membranes via a lipid anchor and has dual function and localization, playing a role in NmMetNI-mediated transport at the inner membrane and moonlighting on the bacterial surface.

Citing Articles

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