Evaluation of CD8 T Cell Subpopulations in Paracoccidioidomycosis

We aimed to verify the frequency of CD8 T cell subsets in patients with acute form and chronic form of paracoccidioidomycosis. Mononuclear cells from paracoccidioidomycosis patients and healthy donors were isolated and phenotyped by flow cytometry. Dendritic cells were pulsed with yeast and co-cultures with lymphocytes. Cytokine production was measured by ELISA. Acute form patients present a higher frequency of Tc1 and Tc10 cells, while chronic form patients have more Tc1 and Tc21 cells, compared with healthy controls. assays showed that induced polarization to the Tc17/Tc22 subsets. Our results suggest that CD8 T cells can respond in a similar way to infection, regardless of the clinical presentation of the disease.