Protocol for Isolating Young Adult Parvalbumin Interneurons from the Mouse Brain for Extraction of High-quality RNA

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2021 Aug 17

PMID 34401780

Citations 5

Authors

Donald J Joseph

Markus von Deimling

Yuiko Hasegawa

Ana G Cristancho

Rashmi Risbud

Almedia J McCoy

Eric D Marsh

Affiliations

Soon will be listed here.

Abstract

Dysfunction in the parvalbumin (PV) subclass of GABAergic interneurons is implicated in several neurodevelopmental disorders that evolve in severity with postnatal developmental stages. Understanding the molecular underpinnings of the postnatal changes in the function of PV interneurons has been limited by the difficulty in the isolation of pure adult PV interneurons and high-quality RNA. Here, we describe our protocol for the isolation of pure young adult PV interneurons and preparation of high-quality RNA from these cells. For complete details on the use and execution of this protocol, please refer to Joseph et al. (2021).

Citing Articles

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SIMS: A deep-learning label transfer tool for single-cell RNA sequencing analysis.

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Loss of postnatal Arx transcriptional activity in parvalbumin interneurons reveals non-cell autonomous disturbances in CA1 pyramidal cells.

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Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq.

Hu N, Gao T, Chen Y Methods Mol Biol. 2024; 2794:341-351.

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Native-state proteomics of Parvalbumin interneurons identifies unique molecular signatures and vulnerabilities to early Alzheimer's pathology.

Kumar P, Goettemoeller A, Espinosa-Garcia C, Tobin B, Tfaily A, Nelson R Nat Commun. 2024; 15(1):2823.

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