Anisakid and Raphidascaridid Parasites in Trachurus Trachurus: Infection Drivers and Possible Effects on the Host's Condition

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2021 Aug 14

PMID 34390382

Citations 2

Authors

Fabio Macchioni

Perla Tedesco

Vanessa Cocca

Andrea Massaro

Paolo Sartor

Alessandro Ligas

Carlo Pretti

Gianfranca Monni

Francesca Cecchi

Monica Caffara

Affiliations

Soon will be listed here.

Abstract

This study investigated the distribution of nematode larvae of Anisakidae and Raphidascarididae (genera Anisakis and Hysterothylacium) in Trachurus trachurus (Linnaeus, 1758) in the Ligurian and central-northern Tyrrhenian Seas. The relationship between the number of parasites and the length and weight parameters of the fish was assessed, and the possible effect of the parasites on the condition factor was evaluated. A total of 190 T. trachurus specimens were collected in July 2019. Parasites were found in 70 individuals. A total of 161 visible larvae were collected in the viscera. Morphological analysis revealed the presence of Anisakis spp. in 55 fish and Hysterothylacium spp. in 15 fish, while 5 fish showed coinfection with both genera. The specimens subjected to PCR (n = 67) showed that 85% of the Anisakis larvae analyzed belonged to the species A. pegreffii, while the remaining 15% belonged to hybrids of A. pegreffii-A. simplex (s.s.). A total of 58% (n = 7) of the Hysterothylacium larvae analyzed belonged to the species H. fabri, while 42% belonged to the species H. aduncum. Our results support the hypothesis that infection with these parasites does not affect the condition of the fish host analyzed, and that body size and depth are major drivers in determining infection levels with Anisakid and Raphidascaridid nematodes.

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References

Abollo E, Gestal C, Pascual S . Anisakis infestation in marine fish and cephalopods from Galician waters: an updated perspective. Parasitol Res. 2001; 87(6):492-9. DOI: 10.1007/s004360100389. View

Abollo E, Paggi L, Pascual S, DAmelio S . Occurrence of recombinant genotypes of Anisakis simplex s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry. Infect Genet Evol. 2003; 3(3):175-81. DOI: 10.1016/s1567-1348(03)00073-x. View

Adroher F, Valero A, Iglesias L . Larval anisakids (Nematoda: Ascaridoidea) in horse mackerel (Trachurus trachurus) from the fish market in Granada (Spain). Parasitol Res. 1996; 82(3):253-6. DOI: 10.1007/s004360050105. View

Angelucci G, Meloni M, Merella P, Sardu F, Madeddu S, Marrosu R . Prevalence of Anisakis spp. and Hysterothylacium spp. larvae in teleosts and cephalopods sampled from waters off Sardinia. J Food Prot. 2011; 74(10):1769-75. DOI: 10.4315/0362-028X.JFP-10-482. View

Balbuena J, Karlsbakk E, Kvenseth A, Saksvik M, Nylund A . Growth and emigration of third-stage larvae of Hysterothylacium aduncum (Nematoda: Anisakidae) in larval herring Clupea harengus. J Parasitol. 2001; 86(6):1271-5. DOI: 10.1645/0022-3395(2000)086[1271:GAEOTS]2.0.CO;2. View

Bush A, Lafferty K, Lotz J, Shostak A . Parasitology meets ecology on its own terms: Margolis et al. revisited. J Parasitol. 1997; 83(4):575-83. View

Cavallero S, Ligas A, Bruschi F, DAmelio S . Molecular identification of Anisakis spp. from fishes collected in the Tyrrhenian Sea (NW Mediterranean). Vet Parasitol. 2012; 187(3-4):563-6. DOI: 10.1016/j.vetpar.2012.01.033. View

Cavallero S, Costa A, Caracappa S, Gambetta B, DAmelio S . Putative hybrids between two Anisakis cryptic species: molecular genotyping using High Resolution Melting. Exp Parasitol. 2014; 146:87-93. DOI: 10.1016/j.exppara.2014.08.017. View

Costa A, Cammilleri G, Graci S, Buscemi M, Vazzana M, Principato D . Survey on the presence of A. simplex s.s. and A. pegreffii hybrid forms in Central-Western Mediterranean Sea. Parasitol Int. 2016; 65(6 Pt A):696-701. DOI: 10.1016/j.parint.2016.08.004. View

10.

DAmelio S, Mathiopoulos K, Santos C, Pugachev O, Webb S, Picanco M . Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. Int J Parasitol. 2000; 30(2):223-6. DOI: 10.1016/s0020-7519(99)00178-2. View

11.

Goffredo E, Azzarito L, Di Taranto P, Mancini M, Normanno G, Didonna A . Prevalence of anisakid parasites in fish collected from Apulia region (Italy) and quantification of nematode larvae in flesh. Int J Food Microbiol. 2019; 292:159-170. DOI: 10.1016/j.ijfoodmicro.2018.12.025. View

12.

Ichalal K, Ramdane Z, Ider D, Kacher M, Iguerouada M, Trilles J . Nematodes parasitizing Trachurus trachurus (L.) and Boops boops (L.) from Algeria. Parasitol Res. 2015; 114(11):4059-68. DOI: 10.1007/s00436-015-4633-6. View

13.

Ishikura H, Kikuchi K, Nagasawa K, Ooiwa T, Takamiya H, Sato N . Anisakidae and anisakidosis. Prog Clin Parasitol. 1993; 3:43-102. DOI: 10.1007/978-1-4612-2732-8_3. View

14.

Lagrue C, Poulin R . Measuring fish body condition with or without parasites: does it matter?. J Fish Biol. 2015; 87(4):836-47. DOI: 10.1111/jfb.12749. View

15.

Manfredi M, Crosa G, Galli P, Ganduglia S . Distribution of Anisakis simplex in fish caught in the Ligurian Sea. Parasitol Res. 2000; 86(7):551-3. DOI: 10.1007/s004360000202. View

16.

Mattiucci S, Nascetti G . Molecular systematics, phylogeny and ecology of anisakid nematodes of the genus Anisakis Dujardin, 1845: an update. Parasite. 2006; 13(2):99-113. DOI: 10.1051/parasite/2006132099. View

17.

Mattiucci S, Cipriani P, Webb S, Paoletti M, Marcer F, Bellisario B . Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). J Parasitol. 2013; 100(2):199-214. DOI: 10.1645/12-120.1. View

18.

Mattiucci S, Cipriani P, Levsen A, Paoletti M, Nascetti G . Molecular Epidemiology of Anisakis and Anisakiasis: An Ecological and Evolutionary Road Map. Adv Parasitol. 2018; 99:93-263. DOI: 10.1016/bs.apar.2017.12.001. View

19.

Mattiucci S, Bello E, Paoletti M, Webb S, Timi J, Levsen A . Novel polymorphic microsatellite loci in and (s. s.) (Nematoda: Anisakidae): implications for species recognition and population genetic analysis. Parasitology. 2019; 146(11):1387-1403. DOI: 10.1017/S003118201900074X. View

20.

Roca-Gerones X, Montoliu I, Godinez-Gonzalez C, Fisa R, Shamsi S . Morphological and genetic characterization of Hysterothylacium Ward & Magath, 1917 (Nematoda: Raphidascarididae) larvae in horse mackerel, blue whiting and anchovy from Spanish Atlantic and Mediterranean waters. J Fish Dis. 2018; 41(10):1463-1475. DOI: 10.1111/jfd.12825. View