Polyoxypregnanes As Safe, Potent, and Specific ABCB1-inhibitory Pro-drugs to Overcome Multidrug Resistance in Cancer Chemotherapy and

Overview

Journal Acta Pharm Sin B

Publisher Elsevier

Specialty Pharmacology

Date 2021 Aug 13

PMID 34386326

Citations 7

Authors

Xu Wu

Chun Yin

Jiang Ma

Stella Chai

Chunyuan Zhang

Sheng Yao

Onat Kadioglu

Thomas Efferth

Yang Ye

Kenneth Kin-Wah To

Ge Lin

Affiliations

Soon will be listed here.

Abstract

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of () were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells inhibiting ABCB1 efflux activity without changing ABCB1 protein expression, which were further identified as specific non-competitive inhibitors of ABCB1 showing multiple binding sites within ABCB1 drug cavity. These POPs did not exhibit ABCB1/drug metabolizing enzymes interplay, and their repeated administration generated predictable pharmacokinetic interaction with paclitaxel without obvious toxicity . We further showed that these POPs enhanced the accumulation of paclitaxel in tumors and overcame ABCB1-mediated chemoresistance. The results suggested that these POPs had the potential to be developed as safe, potent, and specific pro-drugs to reverse ABCB1-mediated MDR. Our work also provided scientific evidence for the use of in combinational chemotherapy.

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