A Pancancer Overview of FBN1, Asprosin and Its Cognate Receptor OR4M1 with Detailed Expression Profiling in Ovarian Cancer

Overview

Journal Oncol Lett

Specialty Oncology

Date 2021 Aug 13

PMID 34386072

Citations 14

Authors

Rachel Kerslake

Marcia Hall

Paola Vagnarelli

Jeyarooban Jeyaneethi

Harpal S Randeva

George Pados

Ioannis Kyrou

Emmanouil Karteris

Affiliations

Soon will be listed here.

Abstract

Ovarian cancer affects >295,000 women worldwide and is the most lethal of gynaecological malignancies. Often diagnosed at a late stage, current research efforts seek to further the molecular understanding of its aetiopathogenesis and the development of novel biomarkers. The present study investigated the expression levels of the glucogenic hormone asprosin [encoded by fibrillin-1 ()], and its cognate receptor, olfactory receptor 4M1 (OR4M1), in ovarian cancer. A blend of open access The Cancer Genome Atlas data, as well as reverse transcription-quantitative PCR (RT-qPCR), immunohistochemistry and immunofluorescence data were used. RT-qPCR revealed expression levels of and in clinical samples and in ovarian cancer cell lines (SKOV-3, PEO1, PEO4 and MDAH-2774), as well as the normal human ovarian surface epithelial cell line (HOSEpiC). Immunohistochemical staining of a tissue microarray was used to identify the expression levels of OR4M1 and asprosin in ovarian cancer samples of varying histological subtype and grade, including clear cell carcinoma, serous ovarian cancer and mucinous adenocarcinoma. Immunofluorescence analysis revealed asprosin expression in SKOV-3 and HOSEpiC cells. These results demonstrated the expression of both asprosin and OR4M1 in normal and malignant human ovarian tissues. This research invokes further investigation to advance the understanding of the role of asprosin and OR4M1 within the ovarian tumour microenvironment.

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References

Millstein J, Budden T, Goode E, Anglesio M, Talhouk A, Intermaggio M . Prognostic gene expression signature for high-grade serous ovarian cancer. Ann Oncol. 2020; 31(9):1240-1250. PMC: 7484370. DOI: 10.1016/j.annonc.2020.05.019. View

Kellenberger L, Bruin J, Greenaway J, Campbell N, Moorehead R, Holloway A . The role of dysregulated glucose metabolism in epithelial ovarian cancer. J Oncol. 2010; 2010:514310. PMC: 2825545. DOI: 10.1155/2010/514310. View

Kocaman N, Artas G . Can novel adipokines, asprosin and meteorin-like, be biomarkers for malignant mesothelioma?. Biotech Histochem. 2019; 95(3):171-175. DOI: 10.1080/10520295.2019.1656344. View

Mo X, Su Z, Yang B, Zeng Z, Lei S, Qiao H . Identification of key genes involved in the development and progression of early-onset colorectal cancer by co-expression network analysis. Oncol Lett. 2020; 19(1):177-186. PMC: 6924089. DOI: 10.3892/ol.2019.11073. View

Guo Q, Song Y, Zhang H, Wu X, Xia P, Dang C . Detection of hypermethylated fibrillin-1 in the stool samples of colorectal cancer patients. Med Oncol. 2013; 30(4):695. DOI: 10.1007/s12032-013-0695-4. View

Du C, Wang C, Guan X, Li J, Du X, Xu Z . Asprosin is associated with anorexia and body fat mass in cancer patients. Support Care Cancer. 2020; 29(3):1369-1375. DOI: 10.1007/s00520-020-05621-8. View

Kerslake R, Hall M, Randeva H, Spandidos D, Chatha K, Kyrou I . Co‑expression of peripheral olfactory receptors with SARS‑CoV‑2 infection mediators: Potential implications beyond loss of smell as a COVID‑19 symptom. Int J Mol Med. 2020; 46(3):949-956. PMC: 7388840. DOI: 10.3892/ijmm.2020.4646. View

Dong D, Dong Y, Fu J, Lu S, Yuan C, Xia M . Bcl2 inhibitor ABT737 reverses the Warburg effect via the Sirt3-HIF1α axis to promote oxidative stress-induced apoptosis in ovarian cancer cells. Life Sci. 2020; 255:117846. DOI: 10.1016/j.lfs.2020.117846. View

Chen J, Cai Y, Xu R, Pan J, Zhou J, Mei J . Identification of four hub genes as promising biomarkers to evaluate the prognosis of ovarian cancer in silico. Cancer Cell Int. 2020; 20:270. PMC: 7315561. DOI: 10.1186/s12935-020-01361-1. View

10.

Wei F, Long A, Wang Y . The Asprosin-OLFR734 hormonal signaling axis modulates male fertility. Cell Discov. 2019; 5:55. PMC: 6868220. DOI: 10.1038/s41421-019-0122-x. View

11.

Aran D, Camarda R, Odegaard J, Paik H, Oskotsky B, Krings G . Comprehensive analysis of normal adjacent to tumor transcriptomes. Nat Commun. 2017; 8(1):1077. PMC: 5651823. DOI: 10.1038/s41467-017-01027-z. View

12.

Summers K, Bokil N, Baisden J, West M, Sweet M, Raggatt L . Experimental and bioinformatic characterisation of the promoter region of the Marfan syndrome gene, FBN1. Genomics. 2009; 94(4):233-40. DOI: 10.1016/j.ygeno.2009.06.005. View

13.

Li W, Zhang H, Guo Q, Wu X, Xu Z, Dang C . Detection of SNCA and FBN1 methylation in the stool as a biomarker for colorectal cancer. Dis Markers. 2015; 2015:657570. PMC: 4353443. DOI: 10.1155/2015/657570. View

14.

Prodoehl M, Hatzirodos N, Irving-Rodgers H, Zhao Z, Painter J, Hickey T . Genetic and gene expression analyses of the polycystic ovary syndrome candidate gene fibrillin-3 and other fibrillin family members in human ovaries. Mol Hum Reprod. 2009; 15(12):829-41. PMC: 2776474. DOI: 10.1093/molehr/gap072. View

15.

Kellenberger L, Petrik J . Hyperglycemia promotes insulin-independent ovarian tumor growth. Gynecol Oncol. 2018; 149(2):361-370. DOI: 10.1016/j.ygyno.2018.02.003. View

16.

Wang C, Lin T, Liu K, Liao C, Liu Y, Wu V . Serum asprosin levels and bariatric surgery outcomes in obese adults. Int J Obes (Lond). 2018; 43(5):1019-1025. DOI: 10.1038/s41366-018-0248-1. View

17.

Hanahan D, Weinberg R . Hallmarks of cancer: the next generation. Cell. 2011; 144(5):646-74. DOI: 10.1016/j.cell.2011.02.013. View

18.

Zhai X, Xue Q, Liu Q, Guo Y, Chen Z . Colon cancer recurrence‑associated genes revealed by WGCNA co‑expression network analysis. Mol Med Rep. 2017; 16(5):6499-6505. PMC: 5865817. DOI: 10.3892/mmr.2017.7412. View

19.

Klimstra W, Heidner H, Johnston R . The furin protease cleavage recognition sequence of Sindbis virus PE2 can mediate virion attachment to cell surface heparan sulfate. J Virol. 1999; 73(8):6299-306. PMC: 112708. DOI: 10.1128/JVI.73.8.6299-6306.1999. View

20.

Naba A, Clauser K, Mani D, Carr S, Hynes R . Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression. Sci Rep. 2017; 7:40495. PMC: 5223159. DOI: 10.1038/srep40495. View