Development of an Anti-infective Coating on the Surface of Intraosseous Implants Responsive to Enzymes and Bacteria

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2021 Aug 13

PMID 34384446

Citations 5

Authors

Xin Liao

Xingfang Yu

Haiping Yu

Jiaqi Huang

Bi Zhang

Jie Xiao

Affiliations

Soon will be listed here.

Abstract

Background: Bacterial proliferation on the endosseous implants surface presents a new threat to the using of the bone implants. Unfortunately, there is no effective constructed antibacterial coating which is bacterial anti-adhesion substrate-independent or have long-term biofilm inhibition functions.

Methods: Drug release effect was tested in Chymotrypsin (CMS) solution and S. aureus. We used bacterial inhibition rate assays and protein leakage experiment to analyze the in vitro antibacterial effect of (Montmorillonite/Poly-L-lysine-Chlorhexidine) [(MMT/PLL-CHX)] multilayer film. We used the CCK-8 assay to analyze the effect of (MMT/PLL-CHX) multilayer films on the growth and proliferation of rat osteoblasts. Rat orthopaedic implant-related infections model was constructed to test the antimicrobial activity effect of (MMT/PLL-CHX) multilayer films in vivo.

Results: In this study, the (MMT/PLL-CHX) multilayer films structure were progressively degraded and showed well concentration-dependent degradation characteristics following incubation with Staphylococcus aureus and CMS solution. Bacterial inhibition rate assays and protein leakage experiment showed high levels of bactericidal activity. While the CCK-8 analysis proved that the (MMT/PLL-CHX) multilayer films possess perfect biocompatibility. It is somewhat encouraging that in the in vivo antibacterial tests, the K-wires coated with (MMT/PLL-CHX) multilayer films showed lower infections incidence and inflammation than the unmodified group, and all parameters are close to SHAM group.

Conclusion: (MMT/PLL-CHX) multilayer films provides a potential therapeutic method for orthopaedic implant-related infections.

Citing Articles

Current Progress and Future Perspectives in Contact and Releasing-Type Antimicrobial Coatings of Orthopaedic Implants: A Systematic Review Analysis Emanated from In Vitro and In Vivo Models.

Kaspiris A, Vasiliadis E, Pantazaka E, Lianou I, Melissaridou D, Savvidis M Infect Dis Rep. 2024; 16(2):298-316.

PMID: 38667751 PMC: 11050497. DOI: 10.3390/idr16020025.

Highly Adhesive Antimicrobial Coatings for External Fixation Devices.

Bredikhin M, Sawant S, Gross C, Antonio E, Borodinov N, Luzinov I Gels. 2023; 9(8).

PMID: 37623093 PMC: 10453896. DOI: 10.3390/gels9080639.

Antibacterial coatings on orthopedic implants.

Chen X, Zhou J, Qian Y, Zhao L Mater Today Bio. 2023; 19:100586.

PMID: 36896412 PMC: 9988588. DOI: 10.1016/j.mtbio.2023.100586.

Smart Bacteria-Responsive Drug Delivery Systems in Medical Implants.

Yang Y, Jiang X, Lai H, Zhang X J Funct Biomater. 2022; 13(4).

PMID: 36278642 PMC: 9589986. DOI: 10.3390/jfb13040173.

Dental plaque-inspired versatile nanosystem for caries prevention and tooth restoration.

Xu Y, You Y, Yi L, Wu X, Zhao Y, Yu J Bioact Mater. 2022; 20:418-433.

PMID: 35784637 PMC: 9233191. DOI: 10.1016/j.bioactmat.2022.06.010.

References

Zheng Y, Wang L, Lu L, Wang Q, Benicewicz B . pH and Thermal Dual-Responsive Nanoparticles for Controlled Drug Delivery with High Loading Content. ACS Omega. 2018; 2(7):3399-3405. PMC: 6044946. DOI: 10.1021/acsomega.7b00367. View

Fernandez I, Busscher H, Metzger S, Grainger D, van der Mei H . Competitive time- and density-dependent adhesion of staphylococci and osteoblasts on crosslinked poly(ethylene glycol)-based polymer coatings in co-culture flow chambers. Biomaterials. 2010; 32(4):979-84. DOI: 10.1016/j.biomaterials.2010.10.011. View

Roldan S, Herrera D, Santa-Cruz I, OConnor A, Gonzalez I, Sanz M . Comparative effects of different chlorhexidine mouth-rinse formulations on volatile sulphur compounds and salivary bacterial counts. J Clin Periodontol. 2004; 31(12):1128-34. DOI: 10.1111/j.1600-051X.2004.00621.x. View

Di Poto A, Sbarra M, Provenza G, Visai L, Speziale P . The effect of photodynamic treatment combined with antibiotic action or host defence mechanisms on Staphylococcus aureus biofilms. Biomaterials. 2009; 30(18):3158-66. DOI: 10.1016/j.biomaterials.2009.02.038. View

Trobos M, Juhlin A, Shah F, Hoffman M, Sahlin H, Dahlin C . In vitro evaluation of barrier function against oral bacteria of dense and expanded polytetrafluoroethylene (PTFE) membranes for guided bone regeneration. Clin Implant Dent Relat Res. 2018; 20(5):738-748. DOI: 10.1111/cid.12629. View

Xu Z, Gao N, Dong S . Preparation and layer-by-layer self-assembly of positively charged multiwall carbon nanotubes. Talanta. 2008; 68(3):753-8. DOI: 10.1016/j.talanta.2005.05.028. View

Unsoy G, Gunduz U . Smart Drug Delivery Systems in Cancer Therapy. Curr Drug Targets. 2016; 19(3):202-212. DOI: 10.2174/1389450117666160401124624. View

Zhang H, Chen Q, Zhang X, Zhu X, Chen J, Zhang H . An Intelligent and Tumor-Responsive Fe Donor and Fe-Dependent Drugs Cotransport System. ACS Appl Mater Interfaces. 2016; 8(49):33484-33498. DOI: 10.1021/acsami.6b11839. View

Soria F, de la Cruz J, Fernandez T, Budia A, Serrano A, Sanchez-Margallo F . Heparin coating in biodegradable ureteral stents does not decrease bacterial colonization-assessment in ureteral stricture endourological treatment in animal model. Transl Androl Urol. 2021; 10(4):1700-1710. PMC: 8100852. DOI: 10.21037/tau-21-19. View

10.

BURT C, Overpeck M . Emergency visits for sports-related injuries. Ann Emerg Med. 2001; 37(3):301-8. DOI: 10.1067/mem.2001.111707. View

11.

Ranalletta M, Rossi L, Barros H, Nally F, Tanoira I, Bongiovanni S . Minimally Invasive Double-Button Fixation of Displaced Lateral Clavicular Fractures in Athletes. Am J Sports Med. 2017; 45(2):462-467. DOI: 10.1177/0363546516666816. View

12.

Canty M, Luke-Marshall N, Campagnari A, Ehrensberger M . Cathodic voltage-controlled electrical stimulation of titanium for prevention of methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii biofilm infections. Acta Biomater. 2016; 48:451-460. DOI: 10.1016/j.actbio.2016.11.056. View

13.

Lucke M, Schmidmaier G, Sadoni S, Wildemann B, Schiller R, Haas N . Gentamicin coating of metallic implants reduces implant-related osteomyelitis in rats. Bone. 2003; 32(5):521-31. DOI: 10.1016/s8756-3282(03)00050-4. View

14.

Thomas E, Mandal S, Ashek-I-Ahmed , Macdonald J, Dane T, Rawle J . Spectroscopic Ellipsometry of Nanocrystalline Diamond Film Growth. ACS Omega. 2019; 2(10):6715-6727. PMC: 6645230. DOI: 10.1021/acsomega.7b00866. View

15.

Bernardi A, Teixeira C . The properties of chlorhexidine and undesired effects of its use in endodontics. Quintessence Int. 2015; 46(7):575-82. DOI: 10.3290/j.qi.a33934. View

16.

Zarkesh N, Nowzari H, Morrison J, Slots J . Tetracycline-coated polytetrafluoroethylene barrier membranes in the treatment of intraosseous periodontal lesions. J Periodontol. 1999; 70(9):1008-16. DOI: 10.1902/jop.1999.70.9.1008. View

17.

Feng Q, Zhang Y, Zhang W, Hao Y, Wang Y, Zhang H . Programmed near-infrared light-responsive drug delivery system for combined magnetic tumor-targeting magnetic resonance imaging and chemo-phototherapy. Acta Biomater. 2016; 49:402-413. DOI: 10.1016/j.actbio.2016.11.035. View

18.

Kuralay F, Dukar N, Bayramli Y . Poly-L-lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection. Electroanalysis. 2020; 30(7):1556-1565. PMC: 7163579. DOI: 10.1002/elan.201800153. View

19.

Gupta M, Martin J, Dollinger B, Hattaway M, Duvall C . Thermogelling, ABC Triblock Copolymer Platform for Resorbable Hydrogels with Tunable, Degradation-Mediated Drug Release. Adv Funct Mater. 2018; 27(47). PMC: 6195316. DOI: 10.1002/adfm.201704107. View

20.

Lee J, Wang H, Kaplan J, Lee W . Effects of Staphylococcus epidermidis on osteoblast cell adhesion and viability on a Ti alloy surface in a microfluidic co-culture environment. Acta Biomater. 2010; 6(11):4422-9. DOI: 10.1016/j.actbio.2010.05.021. View