Expression of the Gene in Adult

The gene in , which encodes a cGMP-dependent protein kinase, is a highly conserved, complex gene with multiple pleiotropic behavioral and physiological functions in both the larval and adult fly. Adult expression is less well characterized than in the larva. We characterized expression in the brain, gastric system, and reproductive systems using a gene-trap allele. In the brain, expression appears to be restricted to multiple sub-types of glia. This glial-specific cellular localization of was supported by single-cell transcriptomic atlases of the adult brain. is extensively expressed in most cell types in the gastric and reproductive systems. We then mapped multiple -regulatory elements responsible for parts of the observed expression patterns by a nested cloned promoter- analysis. The mapped -regulatory elements were consistently modular when comparing the larval and adult expression patterns. These new data using the gene-trap and cloned promoter fusion 's are discussed with respect to previous work using an anti-FOR antibody, which we show here to be non-specific. Future studies of 's function will consider roles for glial subtypes and peripheral tissues (gastric and reproductive systems) in 's pleiotropic behavioral and physiological effects.