Sorbitol and Overexpression Alleviate Temperature Sensitivity in Chaperonin Mutants of

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2021 Aug 11

PMID 34377964

Authors

Aswathy Narayanan

M Anaul Kabir

Affiliations

Soon will be listed here.

Abstract

CCT (Chaperonin containing TCP-1) is a constitutively expressed eukaryotic chaperonin complex involved in the proper folding of proteins like actin and tubulin. Temperature sensitive mutants of CCT complex have been employed in various genetic screens, acting as models to study human CCT, the defects of which are implicated in disease conditions like neurodegeneration. Mutants of CCT complex are sensitive to cell wall stress agents. In this study, we have tested the effects of sorbitol and protein kinase C overexpression on the temperature sensitivity of mutants. We report that both the factors alleviated temperature sensitivity of mutants, indicating the possible role of CCT in maintaining cell wall integrity in .

References

Frydman J, Nimmesgern E, Erdjument-Bromage H, Wall J, Tempst P, Hartl F . Function in protein folding of TRiC, a cytosolic ring complex containing TCP-1 and structurally related subunits. EMBO J. 1992; 11(13):4767-78. PMC: 556952. DOI: 10.1002/j.1460-2075.1992.tb05582.x. View

Lin P, Cardillo T, Richard L, Segel G, Sherman F . Analysis of mutationally altered forms of the Cct6 subunit of the chaperonin from Saccharomyces cerevisiae. Genetics. 1997; 147(4):1609-33. PMC: 1208335. DOI: 10.1093/genetics/147.4.1609. View

Boudiaf-Benmammar C, Cresteil T, Melki R . The cytosolic chaperonin CCT/TRiC and cancer cell proliferation. PLoS One. 2013; 8(4):e60895. PMC: 3628893. DOI: 10.1371/journal.pone.0060895. View

Narayanan A, Pullepu D, Reddy P, Uddin W, Kabir M . Defects in Protein Folding Machinery Affect Cell Wall Integrity and Reduce Ethanol Tolerance in S. cerevisiae. Curr Microbiol. 2016; 73(1):38-45. DOI: 10.1007/s00284-016-1024-x. View

Levin D . Cell wall integrity signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 2005; 69(2):262-91. PMC: 1197416. DOI: 10.1128/MMBR.69.2.262-291.2005. View

Melkani G, Bhide S, Han A, Vyas J, Livelo C, Bodmer R . TRiC/CCT chaperonins are essential for maintaining myofibril organization, cardiac physiological rhythm, and lifespan. FEBS Lett. 2017; 591(21):3447-3458. PMC: 5683924. DOI: 10.1002/1873-3468.12860. View

Kamada Y, Jung U, Piotrowski J, Levin D . The protein kinase C-activated MAP kinase pathway of Saccharomyces cerevisiae mediates a novel aspect of the heat shock response. Genes Dev. 1995; 9(13):1559-71. DOI: 10.1101/gad.9.13.1559. View

Berger J, Berger S, Li M, Jacoby A, Arner A, Bavi N . In Vivo Function of the Chaperonin TRiC in α-Actin Folding during Sarcomere Assembly. Cell Rep. 2018; 22(2):313-322. DOI: 10.1016/j.celrep.2017.12.069. View

RIBAS J, Diaz M, Duran A, Perez P . Isolation and characterization of Schizosaccharomyces pombe mutants defective in cell wall (1-3)beta-D-glucan. J Bacteriol. 1991; 173(11):3456-62. PMC: 207959. DOI: 10.1128/jb.173.11.3456-3462.1991. View

10.

Wright C, Fewell S, Sullivan M, Pipas J, Watkins S, Brodsky J . The Hsp40 molecular chaperone Ydj1p, along with the protein kinase C pathway, affects cell-wall integrity in the yeast Saccharomyces cerevisiae. Genetics. 2007; 175(4):1649-64. PMC: 1855118. DOI: 10.1534/genetics.106.066274. View

11.

Tam S, Geller R, Spiess C, Frydman J . The chaperonin TRiC controls polyglutamine aggregation and toxicity through subunit-specific interactions. Nat Cell Biol. 2006; 8(10):1155-62. PMC: 2829982. DOI: 10.1038/ncb1477. View

12.

Knowlton J, de Castro I, Ashbrook A, Gestaut D, Zamora P, Bauer J . The TRiC chaperonin controls reovirus replication through outer-capsid folding. Nat Microbiol. 2018; 3(4):481-493. PMC: 5874176. DOI: 10.1038/s41564-018-0122-x. View

13.

Shimon L, Hynes G, McCormack E, Willison K, Horovitz A . ATP-induced allostery in the eukaryotic chaperonin CCT is abolished by the mutation G345D in CCT4 that renders yeast temperature-sensitive for growth. J Mol Biol. 2008; 377(2):469-77. DOI: 10.1016/j.jmb.2008.01.011. View

14.

Llorca O, Smyth M, Carrascosa J, Willison K, Radermacher M, Steinbacher S . 3D reconstruction of the ATP-bound form of CCT reveals the asymmetric folding conformation of a type II chaperonin. Nat Struct Biol. 1999; 6(7):639-42. DOI: 10.1038/10689. View

15.

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K . Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila. PLoS Genet. 2019; 15(4):e1008121. PMC: 6508744. DOI: 10.1371/journal.pgen.1008121. View

16.

Kabir M, Sherman F . Overexpressed ribosomal proteins suppress defective chaperonins in Saccharomyces cerevisiae. FEMS Yeast Res. 2008; 8(8):1236-44. DOI: 10.1111/j.1567-1364.2008.00425.x. View