Recovery of Polyphenolic Fraction from Arabica Coffee Pulp and Its Antifungal Applications

Overview

Journal Plants (Basel)

Date 2021 Aug 10

PMID 34371625

Citations 15

Authors

Jiraporn Sangta

Malaiporn Wongkaew

Tibet Tangpao

Patchareeya Withee

Sukanya Haituk

Chaiwat Arjin

Korawan Sringarm

Surat Hongsibsong

Kunrunya Sutan

Tonapha Pusadee

Sarana Rose Sommano

Ratchadawan Cheewangkoon

Affiliations

Soon will be listed here.

Abstract

Coffee pulp is one of the most underutilised by-products from coffee processing. For coffee growers, disposing of this agro-industrial biomass has become one of the most difficult challenges. This study utilised this potential biomass as raw material for polyphenolic antifungal agents. First, the proportion of biomass was obtained from the Arabica green bean processing. The yield of by-products was recorded, and the high-potency biomass was serially extracted with organic solvents for the polyphenol fraction. Quantification of the polyphenols was performed by High Performance Liquid Chromatography (HPLC), then further confirmed by mass spectrometry modes of the liquid chromatography-quadrupole time-of-flight (QTOF). Then, the fraction was used to test antifungal activities against , sp. and . The results illustrated that caffeic acid and epigallocatechin gallate represented in the polyphenol fraction actively inhibited these fungi with an inhibitory concentration (IC50) of 0.09, 0.31 and 0.14, respectively. This study is also the first report on the alternative use of natural biocontrol agent of , the pathogen causing leaf spot in the Arabica coffee.

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References

Arjin C, Hongsibsong S, Pringproa K, Seel-Audom M, Ruksiriwanich W, Sutan K . Effect of Ethanolic Fraction on In Vitro Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus. Vet Sci. 2021; 8(6). PMC: 8229879. DOI: 10.3390/vetsci8060106. View

Patay E, Sali N, Koszegi T, Csepregi R, Balazs V, Nemeth T . Antioxidant potential, tannin and polyphenol contents of seed and pericarp of three Coffea species. Asian Pac J Trop Med. 2016; 9(4):366-371. DOI: 10.1016/j.apjtm.2016.03.014. View

Jiang X, Feng K, Yang X . In vitro Antifungal Activity and Mechanism of Action of Tea Polyphenols and Tea Saponin against Rhizopus stolonifer. J Mol Microbiol Biotechnol. 2015; 25(4):269-76. DOI: 10.1159/000430866. View

Xu B, Chang S . A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci. 2007; 72(2):S159-66. DOI: 10.1111/j.1750-3841.2006.00260.x. View

Wu W, Zhu M, Liang Y, Bai X, Lu Y, Xi J . First Report of Paramyrothecium breviseta Causing Leaf Spot Disease of Coffea canephora in China. Plant Dis. 2021; . DOI: 10.1094/PDIS-10-20-2276-PDN. View

Rodriguez-Carpena J, Morcuende D, Andrade M, Kylli P, Estevez M . Avocado (Persea americana Mill.) phenolics, in vitro antioxidant and antimicrobial activities, and inhibition of lipid and protein oxidation in porcine patties. J Agric Food Chem. 2011; 59(10):5625-35. DOI: 10.1021/jf1048832. View

Martinez-Saez N, Ullate M, Martin-Cabrejas M, Martorell P, Genoves S, Ramon D . A novel antioxidant beverage for body weight control based on coffee silverskin. Food Chem. 2013; 150:227-34. DOI: 10.1016/j.foodchem.2013.10.100. View

Konuk H, Erguden B . Phenolic -OH group is crucial for the antifungal activity of terpenoids via disruption of cell membrane integrity. Folia Microbiol (Praha). 2020; 65(4):775-783. DOI: 10.1007/s12223-020-00787-4. View

Keith L, Velasquez M, Zee F . Identification and Characterization of Pestalotiopsis spp. Causing Scab Disease of Guava, Psidium guajava, in Hawaii. Plant Dis. 2019; 90(1):16-23. DOI: 10.1094/PD-90-0016. View

10.

Chen T, Lu J, Kang B, Lin M, Ding L, Zhang L . Antifungal Activity and Action Mechanism of Ginger Oleoresin Against Isolated From Chinese Olive Fruits. Front Microbiol. 2018; 9:2583. PMC: 6218584. DOI: 10.3389/fmicb.2018.02583. View

11.

Heeger A, Kosinska-Cagnazzo A, Cantergiani E, Andlauer W . Bioactives of coffee cherry pulp and its utilisation for production of Cascara beverage. Food Chem. 2016; 221:969-975. DOI: 10.1016/j.foodchem.2016.11.067. View

12.

Sanchez-Maldonado A, Schieber A, Ganzle M . Antifungal activity of secondary plant metabolites from potatoes (Solanum tuberosum L.): Glycoalkaloids and phenolic acids show synergistic effects. J Appl Microbiol. 2016; 120(4):955-65. DOI: 10.1111/jam.13056. View

13.

Adedapo A, Jimoh F, Afolayan A, Masika P . Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complement Altern Med. 2008; 8:54. PMC: 2566552. DOI: 10.1186/1472-6882-8-54. View

14.

Sommano S, Suppakittpaisarn P, Sringarm K, Junmahasathien T, Ruksiriwanich W . Recovery of Crocins From Floral Tissue of Ellis. Front Nutr. 2020; 7:106. PMC: 7492602. DOI: 10.3389/fnut.2020.00106. View

15.

Rosales Delgado S, Arbelaez A, Rojano B . Antioxidant capacity, bioactive compounds in coffee pulp and implementation in the production of infusions. Acta Sci Pol Technol Aliment. 2019; 18(3):235-248. DOI: 10.17306/J.AFS.0663. View

16.

Matsumoto Y, Kaihatsu K, Nishino K, Ogawa M, Kato N, Yamaguchi A . Antibacterial and antifungal activities of new acylated derivatives of epigallocatechin gallate. Front Microbiol. 2012; 3:53. PMC: 3280433. DOI: 10.3389/fmicb.2012.00053. View

17.

Wisetkomolmat J, Inta A, Krongchai C, Kittiwachana S, Jantanasakulwong K, Rachtanapun P . Ethnochemometric of plants traditionally utilised as local detergents in the forest dependent culture. Saudi J Biol Sci. 2021; 28(5):2858-2866. PMC: 8117162. DOI: 10.1016/j.sjbs.2021.02.018. View

18.

Davis A, Gole T, Baena S, Moat J . The impact of climate change on indigenous Arabica coffee (Coffea arabica): predicting future trends and identifying priorities. PLoS One. 2012; 7(11):e47981. PMC: 3492365. DOI: 10.1371/journal.pone.0047981. View

19.

Khantham C, Yooin W, Sringarm K, Sommano S, Jiranusornkul S, Carmona F . Effects on Steroid 5-Alpha Reductase Gene Expression of Thai Rice Bran Extracts and Molecular Dynamics Study on SRD5A2. Biology (Basel). 2021; 10(4). PMC: 8070419. DOI: 10.3390/biology10040319. View

20.

Wongkaew M, Tinpovong B, Sringarm K, Leksawasdi N, Jantanasakulwong K, Rachtanapun P . Crude Pectic Oligosaccharide Recovery from Thai Chok Anan Mango Peel Using Pectinolytic Enzyme Hydrolysis. Foods. 2021; 10(3). PMC: 7999440. DOI: 10.3390/foods10030627. View