Identification of Essential Genes Associated With Prodigiosin Production in FZSF02

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Aug 9

PMID 34367107

Citations 7

Authors

Xianbo Jia

Fangchen Liu

Ke Zhao

Junjie Lin

Yu Fang

Shouping Cai

Chenqiang Lin

Hui Zhang

Longjun Chen

Jichen Chen

Affiliations

Soon will be listed here.

Abstract

Prodigiosin is a promising secondary metabolite produced mainly by strains. To study the global regulatory mechanism of prodigiosin biosynthesis, a mutagenesis library containing 23,000 mutant clones was constructed with the EZ-Tn5 transposon, and 114 clones in the library showed altered prodigiosin production ability. For 37 of the 114 clones, transposon insertion occurred on the prodigiosin biosynthetic cluster genes; transposon inserted genes of the 77 clones belonged to 33 different outside prodigiosin biosynthetic cluster genes. These 33 genes can be divided into transcription-regulating genes, membrane protein-encoding genes, and metabolism enzyme-encoding genes. Most of the genes were newly reported to be involved in prodigiosin production. Transcriptional levels of the gene were significantly downregulated in 22 mutants with different inserted genes, which was in accordance with the phenotype of decreased prodigiosin production. Functional confirmation of the mutant genes involved in the pyrimidine nucleotide biosynthesis pathway was carried out by adding orotate and uridylate (UMP) into the medium. Gene complementation confirmed the regulatory function of the EnvZ/OmpR two-component regulatory system genes and in prodigiosin production.

Citing Articles

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In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin.

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A comprehensive profiling of quorum quenching by bacterial pigments identifies quorum sensing inhibition and antibiofilm action of prodigiosin against Acinetobacter baumannii.

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PtrA regulates prodigiosin synthesis and biological functions in FZSF02.

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Transcriptional factor OmpR positively regulates prodigiosin biosynthesis in FZSF02 by binding with the promoter of the prodigiosin cluster.

Jia X, Zhao K, Liu F, Lin J, Lin C, Chen J Front Microbiol. 2022; 13:1041146.

PMID: 36466667 PMC: 9712742. DOI: 10.3389/fmicb.2022.1041146.

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