Molecular Characterization of a Ceftriaxone-resistant Neisseria Gonorrhoeae Strain Found in Switzerland: a Case Report

Overview

Journal Ann Clin Microbiol Antimicrob

Publisher Biomed Central

Specialties Infectious Diseases
Microbiology

Date 2021 Aug 7

PMID 34362393

Citations 1

Authors

Konrad Egli

Anna Roditscheff

Ursula Fluckiger

Martin Risch

Lorenz Risch

Thomas Bodmer

Affiliations

Soon will be listed here.

Abstract

Background: The resistance of Neisseria gonorrhoeae to ceftriaxone is unusual in Switzerland. The underlying genotype responsible for resistance is suspected to be novel. Generally, resistance in Neisseria gonorrhoeae (Ng) involves a comprehensive set of genes with many different mutations leading to resistance to different β-lactams and fluoroquinolones.

Case Presentation: A patient had a positive result from specific PCR for Ng. We routinely culture all clinical specimens with a positive NG-PCR. In this particular case, we isolated a strain with resistance to ceftriaxone in Switzerland. A total of seven different genes (penA, ponA, porinB, mtr, gyrA, parC, 23S rRNA gene) in this strain were partially sequenced for comparison with phenotypic susceptibility testing. Interestingly, two different mutations in the porinB gene were observed, and data on this gene are limited. Information on the identified allele type of the penA gene is very limited as well. Three different mutations of parC and gyrA that correlate with ciprofloxacin resistance were found. The combination of ceftriaxone and ciprofloxacin resistance makes an appropriate treatment difficult to obtain due to multidrug resistance.

Conclusion: The combined results for all genes show the appearance of new mutations in central Europe either due to worldwide spread or the emergence of new genetic combinations of mutations.

Citing Articles

Rapid molecular phenotypic antimicrobial susceptibility test for based on propidium monoazide viability PCR.

Tjandra K, Ram-Mohan N, Abe R, Wang T, Yang S bioRxiv. 2023; .

PMID: 36909582 PMC: 10002740. DOI: 10.1101/2023.03.01.530513.

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