Anti-Aging Effect of Urolithin A on Bovine Oocytes

Overview

Journal Animals (Basel)

Date 2021 Aug 7

PMID 34359176

Citations 6

Authors

Elisa Fonseca

Carla Cruz Marques

Jorge Pimenta

Joana Jorge

Maria Conceicao Baptista

Ana Cristina Goncalves

Rosa M L N Pereira

Affiliations

Soon will be listed here.

Abstract

Oxidative stress and mitochondrial dysfunction have been associated with the age-related decline of oocyte quality and strategies for their prevention are currently quested. Urolithin A (UA) is a natural metabolite with pro-apoptotic and antioxidant effects, capable of preventing the accumulation of dysfunctional mitochondria in different aged cells. UA has never been tested in bovine oocytes. Our aim was to study the effect of UA on the developmental potential of cumulus-oocyte-complexes (COCs) and granulosa cells' (GCs) expression of important genes related to reproductive competence. Nuclear maturation progression, mitochondrial membrane potential (MMP) and developmental competence of physiologically mature (22 h) and aged oocytes (30 h of IVM) obtained from prepubertal and adult females, either supplemented with UA or not were assessed. Additionally, the amount of mRNA of several genes , , and ) and the number of DNA copies were quantified in cultured GCs from prepubertal and adult females, either supplemented with UA or not. Our study confirmed the harmful effect of oocyte aging on the nuclear maturation progression, MMP, developmental competence and gene expression levels. UA treatment during maturation enhanced ( < 0.05) the maturation rate and subsequent developmental capacity of aged oocytes. A positive effect ( < 0.05) of UA on physiological maturation, MMP and embryonic development was also identified. UA also interfered on the expression profile of and genes in GCs cultures. Our findings demonstrate that UA supplementation is an effective way to prevent oocyte aging and improves the subsequent bovine embryonic development.

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