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Molecular Characterization of the Gene of Bovine Leukemia Virus in Cattle from Pakistan with NGS-Based Evidence of Virus Heterogeneity

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Journal Pathogens
Date 2021 Aug 6
PMID 34358060
Citations 7
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Abstract

Characterization of the global genetic diversity of the bovine leukemia virus (BLV) is an ongoing international research effort. Up to now BLV sequences have been classified into eleven distinct genotypes. Although BLV genotyping and molecular analysis of field isolates were reported in many countries, there is no report describing BLV genotypes present in cattle from Pakistan. In this study we examined 27 gene sequences from BLV-infected cattle coming from four farms located in Khyber Pakhtunkwa, Gilgit Baltisan and Punjab provinces. Phylogenetic analyses revealed the classification of Pakistani sequences into genotypes G1 and G6. The alignment with the FLK-BLV sequence revealed the presence of 45 mutations, namely, seven in genotype G1 and 33 in genotype G6. Five mutations were found in both, G1 and G6 genotypes. Twelve amino acid substitutions were found in the analyzed sequences, of which only one P264S was specific for sequences from Pakistan. Furthermore, a certain degree of nucleotide heterogeneity was identified by NGS. These results highlight the need for further study on the importance of genetic variability of BLV, especially in the context of its pathogenicity and potential effect on serological detection.

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References
1.
Li H, Durbin R . Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009; 25(14):1754-60. PMC: 2705234. DOI: 10.1093/bioinformatics/btp324. View

2.
Meas S, Usui T, Ohashi K, Sugimoto C, Onuma M . Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds. Vet Microbiol. 2001; 84(3):275-82. DOI: 10.1016/s0378-1135(01)00458-8. View

3.
Erskine R, Bartlett P, Byrem T, Render C, Febvay C, Houseman J . Association between bovine leukemia virus, production, and population age in Michigan dairy herds. J Dairy Sci. 2012; 95(2):727-34. DOI: 10.3168/jds.2011-4760. View

4.
Obenchain V, Lawrence M, Carey V, Gogarten S, Shannon P, Morgan M . VariantAnnotation: a Bioconductor package for exploration and annotation of genetic variants. Bioinformatics. 2014; 30(14):2076-8. PMC: 4080743. DOI: 10.1093/bioinformatics/btu168. View

5.
Balic D, Lojkic I, Periskic M, Bedekovic T, Jungic A, Lemo N . Identification of a new genotype of bovine leukemia virus. Arch Virol. 2012; 157(7):1281-90. DOI: 10.1007/s00705-012-1300-4. View