Genetic Relationships in the Toxin-Producing Fungal Endophyte, Using Polyketide Synthase and Non-Ribosomal Peptide Synthase Genes

Overview

Journal J Fungi (Basel)

Date 2021 Aug 6

PMID 34356917

Citations 4

Authors

Rebecca Creamer

Deana Baucom Hille

Marwa Neyaz

Tesneem Nusayr

Christopher L Schardl

Daniel Cook

Affiliations

Soon will be listed here.

Abstract

The legume hosts a fungal endophyte,  , which produces secondary metabolites (SM), including the toxin swainsonine. Polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) enzymes are associated with biosynthesis of fungal SM. To better understand the origins of the SM, an unannotated genome of was assessed for protein sequences similar to known PKS and NRPS enzymes of fungi. Contigs exhibiting identity with known genes were analyzed at nucleotide and protein levels using available databases. Software were used to identify PKS and NRPS domains and predict identity and function. Confirmation of sequence for selected gene sequences was accomplished using PCR. Thirteen PKS, 5 NRPS, and 4 PKS-NRPS hybrids were identified and characterized with functions including swainsonine and melanin biosynthesis. Phylogenetic relationships among closest amino acid matches with spp. were identified for seven highly conserved PKS and NRPS, including melanin synthesis. Three PKS and NRPS were most closely related to other fungi within the Pleosporaceae family, while five PKS and PKS-NRPS were closely related to fungi in the Pleosporales order. However, seven PKS and PKS-NRPS showed no identity with fungi in the Pleosporales or the class Dothideomycetes, suggesting a different evolutionary origin for those genes.

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