Biodegradation of Deoxynivalenol by Sp. ZHH-013: 3--Deoxynivalenol and 3--Deoxynivalenol As Intermediate Products

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Aug 5

PMID 34349733

Citations 9

Authors

Honghai Zhang

Heng Zhang

Xing Qin

Xiaolu Wang

Yuan Wang

Yao Bin

Xiangming Xie

Fei Zheng

Huiying Luo

Affiliations

Soon will be listed here.

Abstract

Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3--DON was a necessary intermediate product during the conversion from DON to 3--DON. Furthermore, the ZHH-013 strain could also utilize 3--DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3--DON elimination will be further studied, so that new enzymes for DON degradation can be identified.

Citing Articles

Priority actions for Fusarium head blight resistance in durum wheat: Insights from the wheat initiative.

Viviani A, Haile J, Fernando W, Ceoloni C, Kuzmanovic L, Lhamo D Plant Genome. 2025; 18(1):e20539.

PMID: 39757924 PMC: 11701714. DOI: 10.1002/tpg2.20539.

Characterization of deoxynivalenol dehydrogenase from Pelagibacterium sp. SCN 63-126 and its application.

Xu W, Yao J, Ma J, Lu C, Wang C, Sun Y Arch Microbiol. 2024; 207(1):9.

PMID: 39636447 DOI: 10.1007/s00203-024-04208-9.

Biotransformation of Deoxynivalenol by a Dual-Member Bacterial Consortium Isolated from Larval Feces.

Wang Y, Zhao D, Zhang W, Wang S, Huang K, Guo B Toxins (Basel). 2023; 15(8).

PMID: 37624249 PMC: 10467086. DOI: 10.3390/toxins15080492.

Four PQQ-Dependent Alcohol Dehydrogenases Responsible for the Oxidative Detoxification of Deoxynivalenol in a Novel Bacterium D3_3 Originated from the Feces of Larvae.

Wang Y, Zhao D, Zhang W, Wang S, Wu Y, Wang S Toxins (Basel). 2023; 15(6).

PMID: 37368668 PMC: 10301637. DOI: 10.3390/toxins15060367.

Post-Harvest Prevention of Fusariotoxin Contamination of Agricultural Products by Irreversible Microbial Biotransformation: Current Status and Prospects.

Statsyuk N, Popletaeva S, Shcherbakova L BioTech (Basel). 2023; 12(2).

PMID: 37218749 PMC: 10204369. DOI: 10.3390/biotech12020032.

References

Springler A, Hessenberger S, Reisinger N, Kern C, Nagl V, Schatzmayr G . Deoxynivalenol and its metabolite deepoxy-deoxynivalenol: multi-parameter analysis for the evaluation of cytotoxicity and cellular effects. Mycotoxin Res. 2016; 33(1):25-37. PMC: 5239812. DOI: 10.1007/s12550-016-0260-z. View

Lucke A, Doupovec B, Paulsen P, Zebeli Q, Bohm J . Effects of low to moderate levels of deoxynivalenol on feed and water intake, weight gain, and slaughtering traits of broiler chickens. Mycotoxin Res. 2017; 33(4):261-271. PMC: 5644695. DOI: 10.1007/s12550-017-0284-z. View

Bretz M, Beyer M, Cramer B, Knecht A, Humpf H . Thermal degradation of the Fusarium mycotoxin deoxynivalenol. J Agric Food Chem. 2006; 54(17):6445-51. DOI: 10.1021/jf061008g. View

Wang G, Wang Y, Ji F, Xu L, Yu M, Shi J . Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16. Food Chem. 2018; 276:436-442. DOI: 10.1016/j.foodchem.2018.10.011. View

He J, Bondy G, Zhou T, Caldwell D, Boland G, Scott P . Toxicology of 3-epi-deoxynivalenol, a deoxynivalenol-transformation product by Devosia mutans 17-2-E-8. Food Chem Toxicol. 2015; 84:250-9. DOI: 10.1016/j.fct.2015.09.003. View

He C, Fan Y, Liu G, Zhang H . Isolation and identification of a strain of Aspergillus tubingensis with deoxynivalenol biotransformation capability. Int J Mol Sci. 2009; 9(12):2366-2375. PMC: 2635639. DOI: 10.3390/ijms9122366. View

Zhang H, Wang Y, Zhao C, Wang J, Zhang X . Biodegradation of ochratoxin A by Alcaligenes faecalis isolated from soil. J Appl Microbiol. 2017; 123(3):661-668. DOI: 10.1111/jam.13537. View

Fuchs E, Binder E, Heidler D, Krska R . Structural characterization of metabolites after the microbial degradation of type A trichothecenes by the bacterial strain BBSH 797. Food Addit Contam. 2002; 19(4):379-86. DOI: 10.1080/02652030110091154. View

Ito M, Sato I, Ishizaka M, Yoshida S, Koitabashi M, Yoshida S . Bacterial cytochrome P450 system catabolizing the Fusarium toxin deoxynivalenol. Appl Environ Microbiol. 2013; 79(5):1619-28. PMC: 3591976. DOI: 10.1128/AEM.03227-12. View

10.

Juan-Garcia A, Juan C, Tolosa J, Ruiz M . Effects of deoxynivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol on parameters associated with oxidative stress in HepG2 cells. Mycotoxin Res. 2019; 35(2):197-205. DOI: 10.1007/s12550-019-00344-0. View

11.

Yoon S, Ha S, Kwon S, Lim J, Kim Y, Seo H . Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol. 2016; 67(5):1613-1617. PMC: 5563544. DOI: 10.1099/ijsem.0.001755. View

12.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

13.

Karlovsky P . Biological detoxification of the mycotoxin deoxynivalenol and its use in genetically engineered crops and feed additives. Appl Microbiol Biotechnol. 2011; 91(3):491-504. PMC: 3136691. DOI: 10.1007/s00253-011-3401-5. View

14.

Garreau de Loubresse N, Prokhorova I, Holtkamp W, Rodnina M, Yusupova G, Yusupov M . Structural basis for the inhibition of the eukaryotic ribosome. Nature. 2014; 513(7519):517-22. DOI: 10.1038/nature13737. View

15.

Zhang J, Qin X, Guo Y, Zhang Q, Ma Q, Ji C . Enzymatic degradation of deoxynivalenol by a novel bacterium, Pelagibacterium halotolerans ANSP101. Food Chem Toxicol. 2020; 140:111276. DOI: 10.1016/j.fct.2020.111276. View

16.

Ikunaga Y, Sato I, Grond S, Numaziri N, Yoshida S, Yamaya H . Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. Appl Microbiol Biotechnol. 2010; 89(2):419-27. PMC: 3291841. DOI: 10.1007/s00253-010-2857-z. View

17.

Carere J, Hassan Y, Lepp D, Zhou T . The Identification of DepB: An Enzyme Responsible for the Final Detoxification Step in the Deoxynivalenol Epimerization Pathway in 17-2-E-8. Front Microbiol. 2018; 9:1573. PMC: 6056672. DOI: 10.3389/fmicb.2018.01573. View

18.

Li M, Guan E, Bian K . Structure Elucidation and Toxicity Analysis of the Degradation Products of Deoxynivalenol by Gaseous Ozone. Toxins (Basel). 2019; 11(8). PMC: 6723297. DOI: 10.3390/toxins11080474. View

19.

Antonissen G, De Baere S, Devreese M, Van Immerseel F, Martel A, Croubels S . Feed contamination with Fusarium mycotoxins induces a corticosterone stress response in broiler chickens. Poult Sci. 2016; 96(1):14-17. DOI: 10.3382/ps/pew280. View

20.

Bracarense A, Pierron A, Pinton P, Gerez J, Schatzmayr G, Moll W . Reduced toxicity of 3-epi-deoxynivalenol and de-epoxy-deoxynivalenol through deoxynivalenol bacterial biotransformation: In vivo analysis in piglets. Food Chem Toxicol. 2020; 140:111241. DOI: 10.1016/j.fct.2020.111241. View