CircFAM13B Promotes the Proliferation of Hepatocellular Carcinoma by Sponging MiR-212, Upregulating E2F5 Expression and Activating the P53 Pathway

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2021 Aug 5

PMID 34348712

Citations 11

Authors

Ying Xie

Xiaofeng Hang

Wensheng Xu

Jing Gu

Yuanjing Zhang

Jianrong Wang

Xiucui Zhang

Xinghao Cao

Junjie Zhan

Junxue Wang

Jianhe Gan

Affiliations

Soon will be listed here.

Abstract

Background: Most of the biological functions of circular RNAs (circRNAs) and the potential underlying mechanisms in hepatocellular carcinoma (HCC) have not yet been discovered.

Methods: In this study, using circRNA expression data from HCC tumor tissues and adjacent tissues from the Gene Expression Omnibus database, we identified out differentially expressed circRNAs and verified them by qRT-PCT. Functional experiments were performed to evaluate the effects of circFAM13B in HCC in vitro and in vivo.

Results: We found that circFAM13B was the most significantly differentially expressed circRNA in HCC tissue. Subsequently, in vitro and in vivo studies also demonstrated that circFAM13B promoted the proliferation of HCC. Further studies revealed that circFAM13B, a sponge of miR-212, is involved in the regulation of E2F5 gene expression by competitively binding to miR-212, inhibits the activation of the P53 signalling pathway, and promotes the proliferation of HCC cells.

Conclusions: Our findings revealed the mechanism underlying the regulatory role played by circFAM13B, miR-212 and E2F5 in HCC. This study provides a new theoretical basis and novel target for the clinical prevention and treatment of HCC.

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