Calcium Ionophore (A23187) Rescues the Activation of Unfertilized Oocytes After Intracytoplasmic Sperm Injection and Chromosome Analysis of Blastocyst After Activation

Overview

Journal Front Endocrinol (Lausanne)

Specialty Endocrinology

Date 2021 Aug 2

PMID 34335469

Citations 12

Authors

Ziwen Xu

Guidong Yao

Wenbin Niu

Huiying Fan

Xueshan Ma

Senlin Shi

Haixia Jin

Wenyan Song

Yingpu Sun

Affiliations

Soon will be listed here.

Abstract

Calcium is a crucial factor in regulating the biological behavior of cells. The imbalance of calcium homeostasis in cytoplasm will cause abnormal behavior of cells and the occurrence of diseases. In intracytoplasmic sperm injection (ICSI) cycle, the dysfunction of oocyte activation caused by insufficient release of Ca from endoplasmic reticulum is one of the main reasons for repeated fertilization failure. Calcium ionophore (A23187) is a highly selective calcium ionophore, which can form stable complex with Ca and pass through the cell membrane at will, effectively increasing intracellular Ca levels. It has been reported that calcium ionophore (A23187) can activate oocytes and obtain normal embryos. However, there are few studies on unfertilized oocytes after calcium ionophore (A23187) rescue activation in ICSI cycle. The purpose of this study was to analyze the effects of calcium ionophore (A23187) rescue activation on the activation of unfertilized oocytes, embryonic development potential, embryonic development timing and chromosomal aneuploidy, and to compare and analyze the clinical data of patients with calcium ionophore (A23187) activation in clinical application. The results showed that a certain proportion of high-quality blastocysts with normal karyotype could be obtained after calcium ionophore (A23187) rescue activation of unfertilized oocytes, and it did not have a significant effect on the timing of embryo development. In clinical practice, direct activation with calcium ionophore (A23187) after ICSI was better than rescue activation the next day. In conclusions, the studies on the effectiveness and safety of calcium ionophore (A23187) rescue activation for oocytes with ICSI fertilization failure can enable some patients to obtain usable, high-quality embryos during the first ICSI cycle.

Citing Articles

Human oocyte zona pellucida abnormalities: evaluation of clinical impact for different zona pellucida abnormalities and role of using assisted hatching.

Wang H, Yang G, Jiang R, Zhai J, Jin H, Song W J Assist Reprod Genet. 2024; 42(1):303-317.

PMID: 39520601 PMC: 11806172. DOI: 10.1007/s10815-024-03306-3.

Calcium signaling in oocyte quality and functionality and its application.

Chen C, Huang Z, Dong S, Ding M, Li J, Wang M Front Endocrinol (Lausanne). 2024; 15:1411000.

PMID: 39220364 PMC: 11361953. DOI: 10.3389/fendo.2024.1411000.

Phospholipase C zeta: a hidden face of sperm for oocyte activation and early embryonic development.

Azil S, Mbaye M, Louanjli N, Ghazi B, Benkhalifa M Obstet Gynecol Sci. 2024; 67(5):467-480.

PMID: 39086217 PMC: 11424188. DOI: 10.5468/ogs.24019.

Successful Fertilization in a Case of Globozoospermia Through Assisted Oocyte Activation With Calcium Ionophores: A Case Report.

Thapa P, Bawaskar P, Badge A, Pareek C, Kalbande A Cureus. 2024; 16(5):e61065.

PMID: 38916008 PMC: 11195911. DOI: 10.7759/cureus.61065.

Exosomes from uterine fluid promote capacitation of human sperm.

Deng R, Wu Z, He C, Lu C, He D, Li X PeerJ. 2024; 12:e16875.

PMID: 38680889 PMC: 11056104. DOI: 10.7717/peerj.16875.

References

Lu Q, Chen X, Li Y, Zhang X, Liang R, Zhao Y . A live birth of activated one-day-old unfertilized oocyte for a patient who experienced repeatedly near-total fertilization failure after intracytoplasmic sperm injection. Chin Med J (Engl). 2012; 125(3):546-8. View

Zegers-Hochschild F, Crosby J, Musri C, Souza M, Martinez A, Silva A . Assisted reproductive technology in Latin America: the Latin American Registry, 2017. Reprod Biomed Online. 2020; 41(1):44-54. DOI: 10.1016/j.rbmo.2020.02.004. View

Egashira A, Murakami M, Haigo K, Horiuchi T, Kuramoto T . A successful pregnancy and live birth after intracytoplasmic sperm injection with globozoospermic sperm and electrical oocyte activation. Fertil Steril. 2009; 92(6):2037.e5-9. DOI: 10.1016/j.fertnstert.2009.08.013. View

Park K, Song H, Chun S . Late fertilization of unfertilized human oocytes in in vitro fertilization and intracytoplasmic sperm injection cycles: conventional insemination versus ICSI. J Assist Reprod Genet. 2000; 17(8):419-24. PMC: 3455574. DOI: 10.1023/a:1009409100941. View

Ferrer-Buitrago M, Bonte D, Dhaenens L, Vermorgen S, Lu Y, De Sutter P . Assessment of the calcium releasing machinery in oocytes that failed to fertilize after conventional ICSI and assisted oocyte activation. Reprod Biomed Online. 2019; 38(4):497-507. DOI: 10.1016/j.rbmo.2018.12.035. View

Chi H, Koo J, Song S, Lee J, Chang S . Successful fertilization and pregnancy after intracytoplasmic sperm injection and oocyte activation with calcium ionophore in a normozoospermic patient with extremely low fertilization rates in intracytoplasmic sperm injection cycles. Fertil Steril. 2004; 82(2):475-7. DOI: 10.1016/j.fertnstert.2004.01.038. View

Nikiforaki D, Vanden Meerschaut F, De Roo C, Lu Y, Ferrer-Buitrago M, De Sutter P . Effect of two assisted oocyte activation protocols used to overcome fertilization failure on the activation potential and calcium releasing pattern. Fertil Steril. 2015; 105(3):798-806.e2. DOI: 10.1016/j.fertnstert.2015.11.007. View

Nakagawa K, Yamano S, Moride N, Yamashita M, Yoshizawa M, Aono T . Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertil Steril. 2001; 76(1):148-52. DOI: 10.1016/s0015-0282(01)01839-8. View

Ducibella T, Fissore R . The roles of Ca2+, downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development. Dev Biol. 2008; 315(2):257-79. PMC: 4276041. DOI: 10.1016/j.ydbio.2007.12.012. View

10.

Lu Q, Zhao Y, Gao X, Li Y, Ma S, Mullen S . Combination of calcium ionophore A23187 with puromycin salvages human unfertilized oocytes after ICSI. Eur J Obstet Gynecol Reprod Biol. 2005; 126(1):72-6. DOI: 10.1016/j.ejogrb.2005.10.038. View

11.

Economou K, Christopikou D, Tsorva E, Davies S, Mastrominas M, Cazlaris H . The combination of calcium ionophore A23187 and GM-CSF can safely salvage aged human unfertilized oocytes after ICSI. J Assist Reprod Genet. 2016; 34(1):33-41. PMC: 5330978. DOI: 10.1007/s10815-016-0823-0. View

12.

Kuentz P, Vanden Meerschaut F, ElInati E, Nasr-Esfahani M, Gurgan T, Iqbal N . Assisted oocyte activation overcomes fertilization failure in globozoospermic patients regardless of the DPY19L2 status. Hum Reprod. 2013; 28(4):1054-61. DOI: 10.1093/humrep/det005. View

13.

Niu W, Wang L, Xu J, Li Y, Shi H, Li G . Improved clinical outcomes of preimplantation genetic testing for aneuploidy using MALBAC-NGS compared with MDA-SNP array. BMC Pregnancy Childbirth. 2020; 20(1):388. PMC: 7333433. DOI: 10.1186/s12884-020-03082-9. View

14.

Borges Jr E, Braga D, Bonetti T, Iaconelli Jr A, Franco Jr J . Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa. Fertil Steril. 2008; 92(1):131-6. DOI: 10.1016/j.fertnstert.2008.04.046. View

15.

Tejera A, Molla M, Muriel L, Remohi J, Pellicer A, de Pablo J . Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient. Fertil Steril. 2008; 90(4):1202.e1-5. DOI: 10.1016/j.fertnstert.2007.11.056. View

16.

Payne D, Flaherty S, Barry M, Matthews C . Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod. 1997; 12(3):532-41. DOI: 10.1093/humrep/12.3.532. View

17.

Norozi-Hafshejani M, Tavalaee M, Azadi L, Bahadorani M, Nasr-Esfahani M . Effects of assisted oocyte activation with calcium- ionophore and strontium chloride on ICSI outcomes. Iran J Basic Med Sci. 2018; 21(11):1109-1117. PMC: 6251390. DOI: 10.22038/IJBMS.2018.30422.7331. View

18.

Murase Y, Araki Y, Mizuno S, Kawaguchi C, Naito M, Yoshizawa M . Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: case report. Hum Reprod. 2004; 19(7):1604-7. DOI: 10.1093/humrep/deh294. View

19.

Balakier H, Dziak E, Sojecki A, Librach C, Michalak M, Opas M . Calcium-binding proteins and calcium-release channels in human maturing oocytes, pronuclear zygotes and early preimplantation embryos. Hum Reprod. 2002; 17(11):2938-47. DOI: 10.1093/humrep/17.11.2938. View

20.

Gleicher N, Weghofer A, Barad D . Anti-Müllerian hormone (AMH) defines, independent of age, low versus good live-birth chances in women with severely diminished ovarian reserve. Fertil Steril. 2010; 94(7):2824-7. DOI: 10.1016/j.fertnstert.2010.04.067. View