Abnormal Macrophage Polarization in Patients with Myelodysplastic Syndrome

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2021 Aug 2

PMID 34335093

Citations 9

Authors

Gaochao Zhang

Liyan Yang

Yu Han

Haiyue Niu

Li Yan

Zonghong Shao

Limin Xing

Huaquan Wang

Affiliations

Soon will be listed here.

Abstract

Background: This study is aimed at assessing the subsets of bone marrow macrophages in patients with myelodysplastic syndrome (MDS) and exploring the role of macrophages in the pathogenesis of MDS.

Methods: Thirty-eight newly diagnosed MDS patients were enrolled in the Department of Hematology of General Hospital of Tianjin Medical University from June 2015 to June 2016. Bone marrow monocytes and macrophage subsets (M1/M2) were detected in patients with MDS and normal controls by flow cytometry. M1 macrophages were cultured , and the expression of IL-1 and TNF- mRNA was measured using real-time polymerase chain reaction.

Results: Compared with the normal control group, the proportion of bone marrow monocytes was higher (2.11 ± 0.93% vs. 3.66 ± 3.38%), and the mean fluorescence intensity of surface molecule CD14 was lower in the higher-risk (HR) MDS group (639.05 ± 359.78 vs. 458.26 ± 306.72, < 0.05). The ratio of M2 macrophages to monocytes was higher in patients with HR-MDS (1.82 ± 2.47% vs. 3.93 ± 3.81%, < 0.05). The ratio of M1 to M2 macrophages was lower in the HR-MDS group (3.50 ± 3.22 vs. 1.80 ± 0.88, < 0.05). The expression of IL-1 and TNF- mRNA in M1 macrophages was significantly lower in the MDS group ( < 0.05).

Conclusions: Patients with MDS had abnormal macrophage polarization, which may be involved in the alteration of bone marrow microenvironments.

Citing Articles

Assessment of the Monocyte Subpopulations and M1/M2 Macrophage Ratio in Concentrated Bone Marrow Aspirate.

Butler J, Dankert J, Keller L, Azam M, Dahmen J, Kerkhoffs G Cartilage. 2024; :19476035241304308.

PMID: 39651680 PMC: 11626554. DOI: 10.1177/19476035241304308.

Pathogenesis and inflammaging in myelodysplastic syndrome.

Villaume M, Savona M Haematologica. 2024; 110(2):283-299.

PMID: 39445405 PMC: 11788632. DOI: 10.3324/haematol.2023.284944.

Construction and validation of prognostic signatures related to mitochondria and macrophage polarization in gastric cancer.

Zhang Y, Cao J, Yuan Z, Zuo H, Yao J, Tu X Front Oncol. 2024; 14:1433874.

PMID: 39132501 PMC: 11310369. DOI: 10.3389/fonc.2024.1433874.

M2 Polarization May Contribute to Formation of Granulomatous Dermatitis in Progression of Myelodysplastic Syndrome to Acute Myeloid Leukemia.

Fang W, Du J, Su Y, Chiu L, Yang T Dermatol Pract Concept. 2023; 13(4).

PMID: 37992346 PMC: 10656156. DOI: 10.5826/dpc.1304a241.

Arginine metabolism key enzymes affect the prognosis of myelodysplastic syndrome by interfering with macrophage polarization.

Ou Y, Yang Y, Li X, Zhang X, Zhao L, Yang C Cancer Med. 2023; 12(15):16444-16454.

PMID: 37366304 PMC: 10469818. DOI: 10.1002/cam4.6287.

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