Effect of Cryoprotectant Concentration on Bovine Oocyte Permeability and Comparison of Two Membrane Permeability Modelling Approaches

Overview

Journal Sci Rep

Specialty Science

Date 2021 Jul 29

PMID 34321576

Citations 2

Authors

Tania Garcia-Martinez

Teresa Mogas

Steven F Mullen

Iris Martinez-Rodero

Ramila E Gulieva

Adam Z Higgins

Affiliations

Soon will be listed here.

Abstract

The plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification efficiency of bovine oocytes. Our study was designed to determine the concentration-dependent permeability characteristics for immature (GV) and mature (MII) bovine oocytes in the presence of ethylene glycol (EG) and dimethyl sulphoxide (MeSO), and to compare two different modeling approaches: the two parameter (2P) model and a nondilute transport model. Membrane permeability parameters were determined by consecutively exposing oocytes to increasing concentrations of MeSO or EG. Higher water permeability was observed for MII oocytes than GV oocytes in the presence of both MeSO and EG, and in all cases the water permeability was observed to decrease as CPA concentration increased. At high CPA concentrations, the CPA permeability was similar for MeSO and EG, for both MII and GV oocytes, but at low concentrations the EG permeability of GV oocytes was substantially higher. Predictions of cell volume changes during CPA addition and removal indicate that accounting for the concentration dependence of permeability only has a modest effect, but there were substantial differences between the 2P model and the nondilute model during CPA removal, which may have implications for design of improved methods for bovine oocyte vitrification.

Citing Articles

Effects of Cryoprotectant Concentration and Exposure Time during Vitrification of Immature Pre-Pubertal Lamb Cumulus-Oocyte Complexes on Nuclear and Cytoplasmic Maturation.

Temerario L, Martino N, Bennink M, de Wit A, Hiemstra S, DellAquila M Animals (Basel). 2024; 14(16).

PMID: 39199884 PMC: 11350855. DOI: 10.3390/ani14162351.

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking.

Bolton R, Mooney A, Pettit M, Bolton A, Morgan L, Drake G Reprod Fertil. 2022; 3(3):R121-R146.

PMID: 35928671 PMC: 9346332. DOI: 10.1530/RAF-22-0005.

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