Improvement of Fucosylated Oligosaccharides Synthesis by α-L-Fucosidase from Thermotoga Maritima in Water-Organic Cosolvent Reaction System

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2021 Jul 27

PMID 34312785

Authors

Monica A Robles-Arias

Mariano Garcia-Garibay

Sergio Alatorre-Santamaria

Salvador R Tello-Solis

Francisco Guzman-Rodriguez

Lorena Gomez-Ruiz

Gabriela Rodriguez-Serrano

Alma E Cruz-Guerrero

Affiliations

Soon will be listed here.

Abstract

The effects of water activity (a), pH, and temperature on transglycosylation activity of α-L-fucosidase from Thermotoga maritima in the synthesis of fucosylated oligosaccharides were evaluated using different water-organic cosolvent reaction systems. The optimum conditions of transglycosylation reaction were the pH range between 7 and 10 and temperature 90-95 °C. The addition of organic cosolvent decreased α-L-fucosidase transglycosylation activity in the following order: acetone > dimethyl sulfoxide (DMSO) > acetonitrile (0.51 > 0.42 > 0.18 mM/h). However, the presence of DMSO and acetone enhanced enzyme-catalyzed transglycosylation over hydrolysis as demonstrated by the obtained transglycosylation/hydrolysis rate (r) values of 1.21 and 1.43, respectively. The lowest r was calculated for acetonitrile (0.59), though all cosolvents tested improved the transglycosylation rate in comparison to a control assay (0.39). Overall, the study allowed the production of fucosylated oligosaccharides in water-organic cosolvent reaction media using α-L-fucosidase from T. maritima as biocatalyst.

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