Protocol for Resolving Enzyme Orientation and Dynamics in Advanced Porous Materials Via SDSL-EPR

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2021 Jul 26

PMID 34308381

Citations 3

Authors

Yanxiong Pan

Hui Li

Qiaobin Li

Mary Lenertz

Isabelle Schuster

Drew Jordahl

Xiao Zhu

Bingcan Chen

Zhongyu Yang

Affiliations

Soon will be listed here.

Abstract

Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes. For complete information on the generation and use of this protocol, please refer to Pan et al. (2021a).

Citing Articles

Boosting Enzyme Activity in Enzyme Metal-Organic Framework Composites.

Weng Y, Chen R, Hui Y, Chen D, Zhao C Chem Bio Eng. 2024; 1(2):99-112.

PMID: 38566967 PMC: 10983012. DOI: 10.1021/cbe.3c00091.

A Protocol for Custom Biomineralization of Enzymes in Metal-Organic Frameworks (MOFs).

Armstrong Z, Jordahl D, MacRae A, Li Q, Lenertz M, Shen P Bio Protoc. 2024; 14(3):e4930.

PMID: 38379827 PMC: 10875352. DOI: 10.21769/BioProtoc.4930.

A Protocol to Depict the Proteolytic Processes Using a Combination of Metal-Organic Materials (MOMs), Electron Paramagnetic Resonance (EPR), and Mass Spectrometry (MS).

Li Q, Lenertz M, Armstrong Z, MacRae A, Feng L, Ugrinov A Bio Protoc. 2024; 14(1):e4909.

PMID: 38213322 PMC: 10777052. DOI: 10.21769/BioProtoc.4909.

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