Nrf2 Pathway Ameliorates Bladder Dysfunction in Cyclophosphamide-Induced Cystitis Via Suppression of Oxidative Stress

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2021 Jul 26

PMID 34306306

Citations 7

Authors

Bin Ni

Zhengsen Chen

Le Shu

Yunpeng Shao

Yi Huang

Nebiyu Elias Tamrat

Zhongqing Wei

Baixin Shen

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the protective effect and molecular mechanism of nuclear factor E2-related factor 2 (Nrf2) pathway in interstitial cystitis (IC).

Methods: We established a mouse model of IC by cyclophosphamide (CYP) in wild-type mice and Nrf2 gene knockout mice. We examined the histological and functional alterations, the changes of oxidative stress markers, and the expression of the antioxidant genes downstream of Nrf2 pathway.

Results: After CYP administration, the mice showed urinary frequency and urgency, pain sensitization, decreased contractility, bladder edema, and oxidative stress disorder. Notably, the Nrf2 CYP mice had more severe symptoms. The mRNA and protein levels of antioxidant genes downstream of Nrf2 pathway were significantly upregulated in the Nrf2 CYP mice, while there were no significant changes in the Nrf2 CYP mice.

Conclusion: Nrf2 pathway protects bladder injury and ameliorates bladder dysfunction in IC, possibly by upregulating antioxidant genes and inhibiting oxidative stress.

Citing Articles

Dimethyl Fumarate Attenuates Cyclophosphamide-Induced Bladder Damage and Enhances Cytotoxic Activity Against SH-SY5Y Cells.

Barut E, Engin S, Oz E, Reis R J Biochem Mol Toxicol. 2025; 39(3):e70212.

PMID: 40059813 PMC: 11891824. DOI: 10.1002/jbt.70212.

Matched serum- and urine-derived biomarkers of interstitial cystitis/bladder pain syndrome.

Kuret T, Sterle I, Romih R, Veranic P PLoS One. 2024; 19(12):e0309815.

PMID: 39739829 PMC: 11687793. DOI: 10.1371/journal.pone.0309815.

Mechanisms of oxidative stress in interstitial cystitis/bladder pain syndrome.

Mohammad A, Laboulaye M, Shenhar C, Dobberfuhl A Nat Rev Urol. 2024; 21(7):433-449.

PMID: 38326514 DOI: 10.1038/s41585-023-00850-y.

Uroprotective and pain-relieving effect of dietary supplementation with micronized palmitoyl-glucosamine and hesperidin in a chronic model of cyclophosphamide-induced cystitis.

Gugliandolo E, Franco G, Marino Y, Peritore A, Impellizzeri D, Cordaro M Front Vet Sci. 2024; 10:1327102.

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IL-34 attenuates acute T cell-mediated rejection following renal transplantation by upregulating M2 macrophages polarization.

Ni B, Zhang D, Zhou H, Zheng M, Wang Z, Tao J Heliyon. 2024; 10(1):e24028.

PMID: 38230243 PMC: 10789621. DOI: 10.1016/j.heliyon.2024.e24028.

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