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A Four-MicroRNA Panel in Peripheral Blood Identified As an Early Biomarker to Diagnose Acute Myocardial Infarction

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Journal Front Physiol
Date 2021 Jul 26
PMID 34305636
Citations 9
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Abstract

This study aimed to evaluate suitable circulating microRNAs (miRNAs) as diagnostic biomarkers of acute myocardial infarction (AMI). Patients with AMI were enrolled as study participants. All patients with AMI coming from the Second Affiliated Hospital of Nantong University between October 1, 2017 and May 31, 2019 were screened. At the same time, 80 patients with coronary angiographic stenosis <50% during the same period were selected as the control group. Peripheral blood samples were collected at different time points (0, 6, 12, and 24 h after disease onset) to detect the expression of a previously identified promising four-microRNA panel. The expression levels of miRNAs were tested by real-time polymerase chain reaction (RT-PCR), and the receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of circulating miRNAs. Based on the inclusion and exclusion criteria, 80 patients with AMI and 80 controls were enrolled in this study. The expression of circulating miR-1291, miR-217, miR-455-3p, and miR-566 was significantly downregulated in patients with AMI compared with controls. The area under the ROC curve (AUC) of circulating miR-1291, miR-217, miR-455-3p, and miR-566 were 0.82, 0.79, 0.82, and 0.83, respectively. The AUC of these four miRNAs was 0.87 with 83% sensitivity and 87% specificity. The expression peaks of these four miRNAs occurred earlier than those of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB). Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the targets of these four miRNAs were significantly enriched in several signaling pathways associated with AMI progression. Circulating miR-1291, miR-217, miR-455-3p, and miR-566 expression levels were significantly lower in patients with AMI; and combined, this panel of four miRNAs acted as a novel and potential early diagnostic biomarker of AMI.

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References
1.
Cheng Y, Tan N, Yang J, Liu X, Cao X, He P . A translational study of circulating cell-free microRNA-1 in acute myocardial infarction. Clin Sci (Lond). 2010; 119(2):87-95. PMC: 3593815. DOI: 10.1042/CS20090645. View

2.
de Winter R, Koster R, Sturk A, Sanders G . Value of myoglobin, troponin T, and CK-MBmass in ruling out an acute myocardial infarction in the emergency room. Circulation. 1995; 92(12):3401-7. DOI: 10.1161/01.cir.92.12.3401. View

3.
Bialek S, Gorko D, Zajkowska A, Koltowski L, Grabowski M, Stachurska A . Release kinetics of circulating miRNA-208a in the early phase of myocardial infarction. Kardiol Pol. 2015; 73(8):613-9. View

4.
Guo M, Guo L, Weng Y . Implication of peripheral blood miRNA-124 in predicting acute myocardial infarction. Eur Rev Med Pharmacol Sci. 2017; 21(5):1054-1059. View

5.
Zhang R, Lan C, Pei H, Duan G, Huang L, Li L . Expression of circulating miR-486 and miR-150 in patients with acute myocardial infarction. BMC Cardiovasc Disord. 2015; 15:51. PMC: 4466864. DOI: 10.1186/s12872-015-0042-0. View