RNase κ Promotes Robust PiRNA Production by Generating 2',3'-cyclic Phosphate-containing Precursors

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Jul 24

PMID 34301931

Citations 4

Authors

Megumi Shigematsu

Takuya Kawamura

Keisuke Morichika

Natsuko Izumi

Takashi Kiuchi

Shozo Honda

Venetia Pliatsika

Ryuma Matsubara

Isidore Rigoutsos

Susumu Katsuma

Yukihide Tomari

Yohei Kirino

Affiliations

Soon will be listed here.

Abstract

In animal germlines, PIWI proteins and the associated PIWI-interacting RNAs (piRNAs) protect genome integrity by silencing transposons. Here we report the extensive sequence and quantitative correlations between 2',3'-cyclic phosphate-containing RNAs (cP-RNAs), identified using cP-RNA-seq, and piRNAs in the Bombyx germ cell line and mouse testes. The cP-RNAs containing 5'-phosphate (P-cP-RNAs) identified by P-cP-RNA-seq harbor highly consistent 5'-end positions as the piRNAs and are loaded onto PIWI protein, suggesting their direct utilization as piRNA precursors. We identified Bombyx RNase Kappa (BmRNase κ) as a mitochondria-associated endoribonuclease which produces cP-RNAs during piRNA biogenesis. BmRNase κ-depletion elevated transposon levels and disrupted a piRNA-mediated sex determination in Bombyx embryos, indicating the crucial roles of BmRNase κ in piRNA biogenesis and embryonic development. Our results reveal a BmRNase κ-engaged piRNA biogenesis pathway, in which the generation of cP-RNAs promotes robust piRNA production.

Citing Articles

Angiogenin-catalyzed cleavage within tRNA anticodon-loops identified by cP-RNA-seq.

Shigematsu M, Matsubara R, Gumas J, Kawamura T, Kirino Y Biosci Biotechnol Biochem. 2024; 89(3):398-405.

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Led-Seq: ligation-enhanced double-end sequence-based structure analysis of RNA.

Kolberg T, von Lohneysen S, Ozerova I, Wellner K, Hartmann R, Stadler P Nucleic Acids Res. 2023; 51(11):e63.

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What Are the Functional Roles of Piwi Proteins and piRNAs in Insects?.

Santos D, Feng M, Kolliopoulou A, Taning C, Sun J, Swevers L Insects. 2023; 14(2).

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Mammalian antiviral systems directed by small RNA.

Takahashi T, Heaton S, Parrish N PLoS Pathog. 2021; 17(12):e1010091.

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