Anxiolytic Effect of Two Tobacco Essential Oils ( Linn.) on Mice

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jul 24

PMID 34299447

Citations 1

Authors

Danqing Xie

Lei Yao

Yan Huang

Shuaifan Wu

Li Ma

Yuhong Li

Wencui Wang

Affiliations

Soon will be listed here.

Abstract

Tobacco ( Linn.) is a famous traditional herb used in folk medicine. The essential oils of tobacco have been demonstrated in modern studies to possess antioxidant, anti-inflammatory, and neuroprotective properties, while its anxiolytic effect has not been reported. The purpose of this study was to evaluate the anxiolytic effect of Yunnan tobacco essential oil (YTO) and Zimbabwe tobacco essential oil (ZTO) on mice. The constituents of YTO and ZTO were analyzed by GC/MS. The anxiolytic effect of YTO and ZTO (0.1%, 1%, and 10%, /) on male ICR mice was evaluated in the light-dark box test (LDB) and the elevated plus maze test (EPM) test via inhalation and transdermal administration. After the behavioral tests, salivary corticosterone levels in mice were measured. The behavioral analysis showed that the administration of both YTO and ZTO elevated the time that the mice spent in the light chamber in the LDB test compared to the untreated control. In the EPM test, YTO and ZTO increased the time spent in open arms and the number of entries into the open arms. In addition, both YTO and ZTO significantly decreased salivary corticosterone levels in mice ( ≤ 0.001). In summary, our results demonstrated that inhalation and transdermal administration of both YTO and ZTO showed anxiolytic effect on male ICR mice.

Citing Articles

Phytochemicals derived from L. plant contribute to pharmaceutical development.

Zhang W, Pan X, Fu J, Cheng W, Lin H, Zhang W Front Pharmacol. 2024; 15:1372456.

PMID: 38681197 PMC: 11045950. DOI: 10.3389/fphar.2024.1372456.

References

Nohara M, Tohei A, Sato T, Amao H . Evaluation of response to restraint stress by salivary corticosterone levels in adult male mice. J Vet Med Sci. 2016; 78(5):775-80. PMC: 4905830. DOI: 10.1292/jvms.15-0610. View

Yan N, Liu Y, Liu L, Du Y, Liu X, Zhang H . Bioactivities and Medicinal Value of Solanesol and Its Accumulation, Extraction Technology, and Determination Methods. Biomolecules. 2019; 9(8). PMC: 6722674. DOI: 10.3390/biom9080334. View

Fedotova J, Kubatka P, Busselberg D, Shleikin A, Caprnda M, Dragasek J . Therapeutical strategies for anxiety and anxiety-like disorders using plant-derived natural compounds and plant extracts. Biomed Pharmacother. 2017; 95:437-446. DOI: 10.1016/j.biopha.2017.08.107. View

Ayaz M, Sadiq A, Junaid M, Ullah F, Subhan F, Ahmed J . Neuroprotective and Anti-Aging Potentials of Essential Oils from Aromatic and Medicinal Plants. Front Aging Neurosci. 2017; 9:168. PMC: 5447774. DOI: 10.3389/fnagi.2017.00168. View

Lin T, Zhong L, Santiago J . Anti-Inflammatory and Skin Barrier Repair Effects of Topical Application of Some Plant Oils. Int J Mol Sci. 2017; 19(1). PMC: 5796020. DOI: 10.3390/ijms19010070. View

CALATAYUD F, Belzung C, Aubert A . Ethological validation and the assessment of anxiety-like behaviours: methodological comparison of classical analyses and structural approaches. Behav Processes. 2004; 67(2):195-206. DOI: 10.1016/j.beproc.2004.04.002. View

Johnson L, Zuloaga D, Bidiman E, Marzulla T, Weber S, Wahbeh H . ApoE2 Exaggerates PTSD-Related Behavioral, Cognitive, and Neuroendocrine Alterations. Neuropsychopharmacology. 2015; 40(10):2443-53. PMC: 4538360. DOI: 10.1038/npp.2015.95. View

Mohebitabar S, Shirazi M, Bioos S, Rahimi R, Malekshahi F, Nejatbakhsh F . Therapeutic efficacy of rose oil: A comprehensive review of clinical evidence. Avicenna J Phytomed. 2017; 7(3):206-213. PMC: 5511972. View

Zhang N, Zhang L, Feng L, Yao L . Cananga odorata essential oil reverses the anxiety induced by 1-(3-chlorophenyl) piperazine through regulating the MAPK pathway and serotonin system in mice. J Ethnopharmacol. 2018; 219:23-30. DOI: 10.1016/j.jep.2018.03.013. View

10.

Zhang K, Yao L . The anxiolytic effect of Juniperus virginiana L. essential oil and determination of its active constituents. Physiol Behav. 2018; 189:50-58. DOI: 10.1016/j.physbeh.2018.01.004. View

11.

Garlet Q, Rodrigues P, Barbosa L, Londero A, Mello C, Heinzmann B . Nectandra grandiflora essential oil and its isolated sesquiterpenoids minimize anxiety-related behaviors in mice through GABAergic mechanisms. Toxicol Appl Pharmacol. 2019; 375:64-80. DOI: 10.1016/j.taap.2019.05.003. View

12.

Popova V, Ivanova T, Prokopov T, Nikolova M, Stoyanova A, Zheljazkov V . Carotenoid-Related Volatile Compounds of Tobacco ( L.) Essential Oils. Molecules. 2019; 24(19). PMC: 6804150. DOI: 10.3390/molecules24193446. View

13.

Sahib N, Anwar F, Gilani A, Hamid A, Saari N, Alkharfy K . Coriander (Coriandrum sativum L.): a potential source of high-value components for functional foods and nutraceuticals--a review. Phytother Res. 2013; 27(10):1439-56. DOI: 10.1002/ptr.4897. View

14.

Rafii F, Ameri F, Haghani H, Ghobadi A . The effect of aromatherapy massage with lavender and chamomile oil on anxiety and sleep quality of patients with burns. Burns. 2019; 46(1):164-171. DOI: 10.1016/j.burns.2019.02.017. View

15.

Hecht S . Tobacco carcinogens, their biomarkers and tobacco-induced cancer. Nat Rev Cancer. 2003; 3(10):733-44. DOI: 10.1038/nrc1190. View

16.

Bhardwaj M, Sali V, Mani S, Vasanthi H . Neophytadiene from Turbinaria ornata Suppresses LPS-Induced Inflammatory Response in RAW 264.7 Macrophages and Sprague Dawley Rats. Inflammation. 2020; 43(3):937-950. DOI: 10.1007/s10753-020-01179-z. View

17.

Popova V, Ivanova T, Stoyanova A, Nikolova V, Hristeva T, Gochev V . Terpenoids in the Essential Oil and Concentrated Aromatic Products Obtained from L. Leaves. Molecules. 2019; 25(1). PMC: 6983188. DOI: 10.3390/molecules25010030. View

18.

Bourin M, Petit-Demouliere B, Dhonnchadha B, Hascoet M . Animal models of anxiety in mice. Fundam Clin Pharmacol. 2007; 21(6):567-74. DOI: 10.1111/j.1472-8206.2007.00526.x. View

19.

Zhang N, Yao L . Anxiolytic Effect of Essential Oils and Their Constituents: A Review. J Agric Food Chem. 2019; 67(50):13790-13808. DOI: 10.1021/acs.jafc.9b00433. View

20.

Kalueff A, Tuohimaa P . Mouse grooming microstructure is a reliable anxiety marker bidirectionally sensitive to GABAergic drugs. Eur J Pharmacol. 2005; 508(1-3):147-53. DOI: 10.1016/j.ejphar.2004.11.054. View