G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Jul 24

PMID 34299001

Citations 11

Authors

Adriana Volna

Martin Bartas

Vaclav Karlicky

Jakub Nezval

Kristyna Kundratova

Petr Pecinka

Vladimir Spunda

Jiri cerven

Affiliations

Soon will be listed here.

Abstract

G-quadruplexes have long been perceived as rare and physiologically unimportant nucleic acid structures. However, several studies have revealed their importance in molecular processes, suggesting their possible role in replication and gene expression regulation. Pathways involving G-quadruplexes are intensively studied, especially in the context of human diseases, while their involvement in gene expression regulation in plants remains largely unexplored. Here, we conducted a bioinformatic study and performed a complex circular dichroism measurement to identify a stable G-quadruplex in the gene , coding for the RNA polymerase II large subunit. We found that this G-quadruplex-forming locus is highly evolutionarily conserved amongst plants sensu lato (Archaeplastida) that share a common ancestor more than one billion years old. Finally, we discussed a new hypothesis regarding G-quadruplexes interacting with UV light in plants to potentially form an additional layer of the regulatory network.

Citing Articles

The Characterization of G-Quadruplexes in Tobacco Genome and Their Function under Abiotic Stress.

Song K, Li B, Li H, Zhang R, Zhang X, Luan R Int J Mol Sci. 2024; 25(8).

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Spotlight on G-Quadruplexes: From Structure and Modulation to Physiological and Pathological Roles.

DellOca M, Quadri R, Bernini G, Menin L, Grasso L, Rondelli D Int J Mol Sci. 2024; 25(6).

PMID: 38542135 PMC: 10970550. DOI: 10.3390/ijms25063162.

A Molecular Orchestration of Plant Translation under Abiotic Stress.

Suhorukova A, Sobolev D, Milovskaya I, Fadeev V, Goldenkova-Pavlova I, Tyurin A Cells. 2023; 12(20).

PMID: 37887289 PMC: 10605726. DOI: 10.3390/cells12202445.

The plant noncoding transcriptome: a versatile environmental sensor.

Chorostecki U, Bologna N, Ariel F EMBO J. 2023; 42(20):e114400.

PMID: 37735935 PMC: 10577639. DOI: 10.15252/embj.2023114400.

Beyond the Primary Structure of Nucleic Acids: Potential Roles of Epigenetics and Noncanonical Structures in the Regulations of Plant Growth and Stress Responses.

Volna A, Bartas M, Nezval J, Pech R, Pecinka P, Spunda V Methods Mol Biol. 2023; 2642:331-361.

PMID: 36944887 DOI: 10.1007/978-1-0716-3044-0_18.

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