Expression of Human CD Antigens, Including CD1 and CD25, by Human X Mouse Interlineage Leukaemia Hybrids

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1987 Dec 1

PMID 3428925

Citations 1

Authors

G M Taylor

H Morten

T Carr

C Harrison

J Ridway

P Morris Jones

Affiliations

Soon will be listed here.

Abstract

The expression of human cell-membrane antigens by hybrid cell lines derived by fusing a human B-ALL and mouse BW 5147 T-lymphoma cells has been studied. Using monoclonal antibodies (mAb), the phenotypes of 19 of the 24 hybrids which grew 11-44 days post-fusion have been analysed by indirect membrane immunofluorescence (IF). These uncloned hybrid cells were assayed early after outgrowth, prior to extensive human chromosome and antigen loss. Nonetheless, cytogenetic analysis showed that all hybrids contained variable numbers of human chromosomes. Phenotypic analysis showed that the hybrids could be grouped as follows: a high frequency expressing CD25 (IL-2 receptor), human T200, HLA class I alpha and beta 2microglobulin, and reacting with the mAb H207 and 12E7; an intermediate frequency expressing CD1 and CD2; and a low frequency expressing CD3, CD4, CD5, CD7, CD8 and CD9. This pattern of antigen expression resembled the frequency of these cells in the human B-ALL parent line. Cell sorting was used to immunoselect hybrids expressing CD1 and CD2, but CD1 expression was unstable during subsequent culture.

Citing Articles

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PMID: 2228027 PMC: 1384314.

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