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Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle That is Blocked by Antiviral Substance(s)

Overview
Journal Bio Protoc
Specialty Biology
Date 2021 Jul 21
PMID 34286039
Citations 15
Authors
Chie Aoki-Utsubo
Ming Chen
Hak Hotta
Affiliations
Soon will be listed here.
Abstract

Viruses infect their host cells to produce progeny virus particles through the sequential steps of the viral life cycle, such as viral attachment, entry, penetration and post-entry events. This protocol describes time-of-addition and temperature-shift assays that are employed to explore which step(s) in the viral life cycle is blocked by an antiviral substance(s).

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