Delphinidin-3-sambubioside from Hibiscus Sabdariffa. L Attenuates Hyperlipidemia in High Fat Diet-induced Obese Rats and Oleic Acid-induced Steatosis in HepG2 Cells

Overview

Journal Bioengineered

Date 2021 Jul 20

PMID 34281481

Citations 9

Authors

Qionghua Long

Hongyan Chen

Wenhui Yang

Li Yang

Lijuan Zhang

Affiliations

Soon will be listed here.

Abstract

. L is folk medicine that is often used for its hypolipidemic and antihypertensive effects; however, the active compound responsible for its anti-obesity effect is presently unknown. Delphinidin-3-sambubioside (Dp3-Sam) is an anthocyanin, was extracted from L. The present research aimed to investigate the role of Dp3-Sam in the prevention of hyperlipidemia in vivo and in vitro. Rats were fed with a standard chow diet (Control group) or high-fat diet (HFD and DP group) for eight weeks. Besides, HepG2 cells were stimulated with 0.2 mM oleic acid, with or without Dp3-Sam (100-200 µg/ml). Lipid profiles were measured by commercial kits. Oil Red O staining was performed to measure the hepatic and intracellular lipid levels. The key genes of lipid metabolism were measured by RT-PCR. In HFD-fed rats, Dp3-Sam reduced the body weight gain, visceral fat, and abdominal fat and decreased hepatic lipid deposits. Dp3-Sam decreased intracellular TG levels and lipid accumulation in oleic acid-treated HepG2 cells. Besides, Dp3-Sam downregulated the mRNA expression of HMG-CoA reductase (), sterol regulatory element-binding protein-1 c (), fatty acid synthase (), and acetyl-CoA carboxylase () and upregulated the mRNA expression of cholesterol 7α-hydroxylase (), carnitine palmitoyltransferase1 (), acyl-coenzyme A oxidase (), and peroxisome proliferator-activated receptor alpha (). Dp3-Sam up-regulated the expression of phosphorylation of AMP-activated protein kinase (pAMPK) in HFD-fed rats. Our findings indicated that Dp3-Sam possesses the potential to improve lipid metabolism dysfunction and our results offered evidence for the use of Dp3-Sam as therapy for the prevention of obesity and dyslipidemia.

Citing Articles

Impact of Dapagliflozin on Hepatic Lipid Metabolism and a Dynamic Model of Ketone Body Levels.

Wan Z, Yuan M, Liu Z, Cai Y, He H, Hao K AAPS J. 2025; 27(1):38.

PMID: 39900889 DOI: 10.1208/s12248-025-01024-x.

Targets and Effects of Common Biocompounds of (Delphinidin-3-Sambubiosid, Quercetin, and Hibiscus Acid) in Different Pathways of Human Cells According to a Bioinformatic Assay.

Zuniga-Hernandez S, Garcia-Iglesias T, Macias-Carballo M, Perez-Larios A, Gutierrez-Mercado Y, Camargo-Hernandez G Nutrients. 2024; 16(4).

PMID: 38398890 PMC: 10893457. DOI: 10.3390/nu16040566.

Obesogenic polystyrene microplastic exposures disrupt the gut-liver-adipose axis.

Zhao J, Adiele N, Gomes D, Malovichko M, Conklin D, Ekuban A Toxicol Sci. 2024; 198(2):210-220.

PMID: 38291899 PMC: 10964747. DOI: 10.1093/toxsci/kfae013.

Regulation and targeting of SREBP-1 in hepatocellular carcinoma.

Su F, Koeberle A Cancer Metastasis Rev. 2023; 43(2):673-708.

PMID: 38036934 PMC: 11156753. DOI: 10.1007/s10555-023-10156-5.

In Vitro Antiprotozoal Activity of Extract against a Ciliate Causing High Mortalities in Turbot Aquaculture.

Carvalho A, Domingues I, Carvalho C, Silva A, Soares A, Marques C Biology (Basel). 2023; 12(7).

PMID: 37508344 PMC: 10376481. DOI: 10.3390/biology12070912.

References

Daval M, Foufelle F, Ferre P . Functions of AMP-activated protein kinase in adipose tissue. J Physiol. 2006; 574(Pt 1):55-62. PMC: 1817807. DOI: 10.1113/jphysiol.2006.111484. View

Li Z, Wu Y, Yu B . Methylophiopogonanone A, an Ophiopogon homoisoflavonoid, alleviates high-fat diet-induced hyperlipidemia: assessment of its potential mechanism. Braz J Med Biol Res. 2020; 53(3):e9201. PMC: 7057930. DOI: 10.1590/1414-431X20199201. View

Garcia D, Hellberg K, Chaix A, Wallace M, Herzig S, Badur M . Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Cell Rep. 2019; 26(1):192-208.e6. PMC: 6344045. DOI: 10.1016/j.celrep.2018.12.036. View

Choi W, Gwon S, Ahn J, Jung C, Ha T . Cooked rice prevents hyperlipidemia in hamsters fed a high-fat/cholesterol diet by the regulation of the expression of hepatic genes involved in lipid metabolism. Nutr Res. 2013; 33(7):572-9. DOI: 10.1016/j.nutres.2013.04.006. View

Wang Q, Liu S, Zhai A, Zhang B, Tian G . AMPK-Mediated Regulation of Lipid Metabolism by Phosphorylation. Biol Pharm Bull. 2018; 41(7):985-993. DOI: 10.1248/bpb.b17-00724. View

Zhu K, Nie S, Tan L, Li C, Gong D, Xie M . A Polysaccharide from Ganoderma atrum Improves Liver Function in Type 2 Diabetic Rats via Antioxidant Action and Short-Chain Fatty Acids Excretion. J Agric Food Chem. 2016; 64(9):1938-44. DOI: 10.1021/acs.jafc.5b06103. View

. Worldwide trends in diabetes since 1980: a pooled analysis of 751 population-based studies with 4.4 million participants. Lancet. 2016; 387(10027):1513-1530. PMC: 5081106. DOI: 10.1016/S0140-6736(16)00618-8. View

Haerer W, Delbaere K, Bartlett H, Lord S, Rowland J . Relationships between HMG-CoA reductase inhibitors (statin) use and strength, balance and falls in older people. Intern Med J. 2011; 42(12):1329-34. DOI: 10.1111/j.1445-5994.2011.02622.x. View

Khera R, Murad M, Chandar A, Dulai P, Wang Z, Prokop L . Association of Pharmacological Treatments for Obesity With Weight Loss and Adverse Events: A Systematic Review and Meta-analysis. JAMA. 2016; 315(22):2424-34. PMC: 5617638. DOI: 10.1001/jama.2016.7602. View

10.

Ojulari O, Lee S, Nam J . Beneficial Effects of Natural Bioactive Compounds from L. on Obesity. Molecules. 2019; 24(1). PMC: 6337177. DOI: 10.3390/molecules24010210. View

11.

Huang W, Chen Y, Liu H, Wu S, Liou C . Ginkgolide C reduced oleic acid-induced lipid accumulation in HepG2 cells. Saudi Pharm J. 2018; 26(8):1178-1184. PMC: 6260475. DOI: 10.1016/j.jsps.2018.07.006. View

12.

Pan H, Fu C, Huang L, Jiang Y, Deng X, Guo J . Anti-Obesity Effect of Chitosan Oligosaccharide Capsules (COSCs) in Obese Rats by Ameliorating Leptin Resistance and Adipogenesis. Mar Drugs. 2018; 16(6). PMC: 6025060. DOI: 10.3390/md16060198. View

13.

Chen X, Li X, Zhang X, You L, Cheung P, Huang R . Antihyperglycemic and antihyperlipidemic activities of a polysaccharide from Physalis pubescens L. in streptozotocin (STZ)-induced diabetic mice. Food Funct. 2019; 10(8):4868-4876. DOI: 10.1039/c9fo00687g. View

14.

Li Y, Xu S, Mihaylova M, Zheng B, Hou X, Jiang B . AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice. Cell Metab. 2011; 13(4):376-388. PMC: 3086578. DOI: 10.1016/j.cmet.2011.03.009. View

15.

Tanaka K, Nakanishi T . Obesity as a risk factor for various diseases: necessity of lifestyle changes for healthy aging. Appl Human Sci. 1996; 15(4):139-48. DOI: 10.2114/jpa.15.139. View

16.

Riaz G, Chopra R . A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomed Pharmacother. 2018; 102:575-586. DOI: 10.1016/j.biopha.2018.03.023. View

17.

Yu Q, Liu Y, Wu Y, Chen Y . Dihydrocurcumin ameliorates the lipid accumulation, oxidative stress and insulin resistance in oleic acid-induced L02 and HepG2 cells. Biomed Pharmacother. 2018; 103:1327-1336. DOI: 10.1016/j.biopha.2018.04.143. View

18.

Ziamajidi N, Khaghani S, Hassanzadeh G, Vardasbi S, Ahmadian S, Nowrouzi A . Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPARα and SREBP-1. Food Chem Toxicol. 2013; 58:198-209. DOI: 10.1016/j.fct.2013.04.018. View

19.

Assifi M, Suchankova G, Constant S, Prentki M, Saha A, Ruderman N . AMP-activated protein kinase and coordination of hepatic fatty acid metabolism of starved/carbohydrate-refed rats. Am J Physiol Endocrinol Metab. 2005; 289(5):E794-800. DOI: 10.1152/ajpendo.00144.2005. View

20.

Despres J, Lemieux I . Abdominal obesity and metabolic syndrome. Nature. 2006; 444(7121):881-7. DOI: 10.1038/nature05488. View