Single-cell Analysis of Human Skin Identifies CD14+ Type 3 Dendritic Cells Co-producing IL1B and IL23A in Psoriasis

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2021 Jul 19

PMID 34279540

Citations 53

Authors

Satoshi Nakamizo

Charles-Antoine Dutertre

Ahad Khalilnezhad

Xiao Meng Zhang

Shawn Lim

Josephine Lum

Geraldine Koh

Charlene Foong

Pearly Jean Ai Yong

Kahbing Jasmine Tan

Reiko Sato

Kaori Tomari

Laurent Yvan-Charvet

Helen He

Emma Guttman-Yassky

Benoit Malleret

Rintaro Shibuya

Masashi Iwata

Baptiste Janela

Tsuyoshi Goto

Tan Siyun Lucinda

Mark B Y Tang

Colin Theng

Valerie Julia

Feriel Hacini-Rachinel

Kenji Kabashima

Florent Ginhoux

Affiliations

Soon will be listed here.

Abstract

Inflammatory skin diseases including atopic dermatitis (AD) and psoriasis (PSO) are underpinned by dendritic cell (DC)-mediated T cell responses. Currently, the heterogeneous human cutaneous DC population is incompletely characterized, and its contribution to these diseases remains unclear. Here, we performed index-sorted single-cell flow cytometry and RNA sequencing of lesional and nonlesional AD and PSO skin to identify macrophages and all DC subsets, including the newly described mature LAMP3+BIRC3+ DCs enriched in immunoregulatory molecules (mregDC) and CD14+ DC3. By integrating our indexed data with published skin datasets, we generated a myeloid cell universe of DC and macrophage subsets in healthy and diseased skin. Importantly, we found that CD14+ DC3s increased in PSO lesional skin and co-produced IL1B and IL23A, which are pathological in PSO. Our study comprehensively describes the molecular characteristics of macrophages and DC subsets in AD and PSO at single-cell resolution, and identifies CD14+ DC3s as potential promoters of inflammation in PSO.

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