The Total MRNA Concentration Buffering System in Yeast is Global Rather Than Gene-specific

Overview

Journal RNA

Specialty Molecular Biology

Date 2021 Jul 17

PMID 34272303

Citations 9

Authors

Jose Garcia-Martinez

Daniel A Medina

Pablo Bellvis

Mai Sun

Patrick Cramer

Sebastian Chavez

Jose E Perez-Ortin

Affiliations

Soon will be listed here.

Abstract

Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast We also assessed mRNA concentrations and their synthesis rates in nonsense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one.

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